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Anti-PKC alpha antibody [133] (ab11723)

Key features and details

  • Mouse monoclonal [133] to PKC alpha
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human
  • Isotype: IgG2a

Overview

  • Product name

    Anti-PKC alpha antibody [133]
    See all PKC alpha primary antibodies

  • Description

    Mouse monoclonal [133] to PKC alpha

  • Host species

    Mouse

  • Specificity

    ab11723 recognises the alpha isoform of PKC. This antibody recognises the sequence PQFVHPILQSAV at the C terminus of PKC alpha.

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Full length protein corresponding to PKC alpha.

  • General notes

    This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

Properties

Images

  • Western blot - Anti-PKC alpha antibody [133] (ab11723)
    Western blot - Anti-PKC alpha antibody [133] (ab11723)

    Lanes 1, 5 and 9: Wild-type HAP1 cell lysate (20 µg)
    Lanes 2, 6 and 10: PKC alpha knockout HAP1 cell lysate (20 µg)
    Lanes 3, 7 and 11: K562 cell lysate (20 µg)
    Lanes 4, 8 and 12: HEK293 cell lysate (20 µg)
    Lanes 1, 2, 3 and 4: Green signal from target – ab11723 observed at 77 kDa
    Lanes 5, 6, 7 and 8: Red signal from loading control – ab8245 observed at 37 kDa
    Lanes 9, 10, 11 and 12: Merged (red and green) signal

    ab11723 was shown to specifically react with PKC alpha in wild-type HAP1 cells. No band was observed when PKC alpha knockout samples were examined. Wild-type and PKC alpha knockout samples were subjected to SDS-PAGE. ab11723 and ab8245 (loading control to GAPDH) were diluted 1/500 and 1/2000 respectively and incubated overnight at 4ºC. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-PKC alpha antibody [133] (ab11723)
    Western blot - Anti-PKC alpha antibody [133] (ab11723)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: PKC alpha knockout HAP1 cell lysate (20 µg)
    Lane 3: K562 cell lysate (20 µg)
    Lane 4: HEK293 cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab11723 observed at 77 kDa. Red - loading control, ab8245, observed at 37 kDa.

    This western blot image is a comparison between ab11723 and a competitor's top cited rabbit polyclonal antibody.

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