Anti-PKC alpha antibody [133] (ab11723)
![Anti-PKC alpha antibody [133] (ab11723)](https://www.abcam.com/ps/products/11/ab11723/Images/ab11723-252500-anti-pkc-alpha-antibody-133-western-blot.jpg "Anti-PKC alpha antibody [133] (ab11723)")
Key features and details
- Mouse monoclonal [133] to PKC alpha
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-PKC alpha antibody [133]
See all PKC alpha primary antibodies -
Description
Mouse monoclonal [133] to PKC alpha -
Host species
Mouse -
Specificity
ab11723 recognises the alpha isoform of PKC. This antibody recognises the sequence PQFVHPILQSAV at the C terminus of PKC alpha. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Full length protein corresponding to PKC alpha.
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General notes
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
133 -
Myeloma
Sp2/0 -
Isotype
IgG2a -
Research areas
Images
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Western blot - Anti-PKC alpha antibody [133] (ab11723)
Lanes 1, 5 and 9: Wild-type HAP1 cell lysate (20 µg)
Lanes 2, 6 and 10: PKC alpha knockout HAP1 cell lysate (20 µg)
Lanes 3, 7 and 11: K562 cell lysate (20 µg)
Lanes 4, 8 and 12: HEK293 cell lysate (20 µg)
Lanes 1, 2, 3 and 4: Green signal from target – ab11723 observed at 77 kDa
Lanes 5, 6, 7 and 8: Red signal from loading control – ab8245 observed at 37 kDa
Lanes 9, 10, 11 and 12: Merged (red and green) signalab11723 was shown to specifically react with PKC alpha in wild-type HAP1 cells. No band was observed when PKC alpha knockout samples were examined. Wild-type and PKC alpha knockout samples were subjected to SDS-PAGE. ab11723 and ab8245 (loading control to GAPDH) were diluted 1/500 and 1/2000 respectively and incubated overnight at 4ºC. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-PKC alpha antibody [133] (ab11723)](https://www.abcam.com/ps/products/11/ab11723/Images/ab11723-277462-anti-pkc-alpha-antibody-133-western-blot.jpg)
Western blot - Anti-PKC alpha antibody [133] (ab11723)Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: PKC alpha knockout HAP1 cell lysate (20 µg)
Lane 3: K562 cell lysate (20 µg)
Lane 4: HEK293 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab11723 observed at 77 kDa. Red - loading control, ab8245, observed at 37 kDa.This western blot image is a comparison between ab11723 and a competitor's top cited rabbit polyclonal antibody.
