Anti-PKC alpha (phospho T638) antibody [E195] (ab32502)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [E195] to PKC alpha (phospho T638)
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-PKC alpha (phospho T638) antibody [E195]
See all PKC alpha primary antibodies -
Description
Rabbit monoclonal [E195] to PKC alpha (phospho T638) -
Host species
Rabbit -
Specificity
The antibody only detects PKC alpha phosphorylated on Threonine 638. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human -
Immunogen
A synthetic phospho-peptide corresponding to residues surrounding Thr638 of human PKC alpha.
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Positive control
- 293 cell lysate and normal brain tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
E195 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-PKC alpha (phospho T638) antibody [E195] (ab32502) at 1/20000 dilution
Lane 1 : 293 cell lysate, untreated
Lane 2 : 293 cell lysate, treated with TPA.
Predicted band size: 77 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
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Immunohistochemical staining of untreated paraffin-embedded normal brain tissue using ab32502 at 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical staining of Phosphatase-treated paraffin-embedded normal brain tissue using ab32502 at 1/250 dilution
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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