Call: +7 771 977 66 65, +7 705 421 2277
Sign in or Register
My basket

Astana Biomed Group, an authorized Abcam distributor in Central Asia

Abiomed homepage

  • Categories
    Signal Transduction
    Cancer
    Epigenetics and Nuclear Signaling
    Immunology
    Cell Biology
    Cardiovascular
    Neuroscience
    Tags & Cell Markers
    Kits/ Lysates/ Other
    Developmental Biology
    Microbiology
    Biochemicals
    Secondary antibodies
    Isotype/Loading Controls
    Antibody Arrays
  • About us
  • Partners
  • Contact
    Address

    Saryarka 32, 18, 010000, Astana city, Kazakhstan

    Telephone +7 771 977 66 65, +7 705 421 2277

    Email

    laboratory@ctlab.kz, orders@abiomed.kz

Back to category
Signal Transduction Protein Phosphorylation Ser / Thr Kinases PKC

Anti-PKC alpha (phospho T638) antibody [E195] (ab32502)

Price and availability

311 587 ₸

Availability

Order now and get it on Wednesday March 10, 2021

Anti-PKC alpha (phospho T638) antibody [E195] (ab32502)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [E195] to PKC alpha (phospho T638)
  • Suitable for: WB, IHC-P
  • Reacts with: Human

You may also be interested in

Product image
Anti-PKC beta 1 antibody [EPR18512] - BSA and Azide free (ab232518)
Product image
Anti-DGKZ/DGK-zeta antibody [EPR22040-72] - BSA and Azide free (ab239792)
Product image
Anti-MARCKS (phospho S158) antibody [EP2113Y] (ab81295)
Product image
Anti-H2R antibody [EPR5630] (ab108540)

Overview

  • Product name

    Anti-PKC alpha (phospho T638) antibody [E195]
    See all PKC alpha primary antibodies
  • Description

    Rabbit monoclonal [E195] to PKC alpha (phospho T638)
  • Host species

    Rabbit
  • Specificity

    The antibody only detects PKC alpha phosphorylated on Threonine 638.
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    A synthetic phospho-peptide corresponding to residues surrounding Thr638 of human PKC alpha.

  • Positive control

    • 293 cell lysate and normal brain tissue.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    E195
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Ser / Thr Kinases
    • PKC
    • Cancer
    • Signal transduction
    • Protein phosphorylation
    • Serine/threonine kinases
    • Other
    • Cardiovascular
    • Heart
    • Hypertrophy
    • Other

Images

  • Western blot - Anti-PKC alpha (phospho T638) antibody [E195] (ab32502)
    Western blot - Anti-PKC alpha (phospho T638) antibody [E195] (ab32502)
    All lanes : Anti-PKC alpha (phospho T638) antibody [E195] (ab32502) at 1/20000 dilution

    Lane 1 : 293 cell lysate, untreated
    Lane 2 : 293 cell lysate, treated with TPA.

    Predicted band size: 77 kDa
    Observed band size: 80 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC alpha (phospho T638) antibody [E195] (ab32502)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC alpha (phospho T638) antibody [E195] (ab32502)

    Immunohistochemical staining of untreated paraffin-embedded normal brain tissue using ab32502 at 1/250 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC alpha (phospho T638) antibody [E195] (ab32502)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC alpha (phospho T638) antibody [E195] (ab32502)

    Immunohistochemical staining of Phosphatase-treated paraffin-embedded normal brain tissue using ab32502 at 1/250 dilution

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-PKC alpha (phospho T638) antibody [E195] (ab32502)
    Anti-PKC alpha (phospho T638) antibody [E195] (ab32502)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-PKC alpha (phospho T638) antibody [E195] (ab32502)

  •  
  • Product image

    Anti-PKC alpha (phospho T497) antibody [EP2608Y] (ab76016)

    Applications: Dot, IHC-P, WB

  •  
  • Product image

    Anti-PKC alpha (phospho T497) antibody (ab226785)

    Applications: WB

  •  
  • Product image

    Anti-PKC alpha antibody [Y124] (ab32376)

    Applications: Flow Cyt, ICC/IF, IHC-P, IP, WB

  •  
  • Product image

    HRP Anti-PKC alpha antibody [Y124] (ab205422)

    Applications: IHC-P, WB

  •  
  • Product image

    Anti-PKC alpha antibody [Y124] - BSA and Azide free (ab221611)

    Applications: Flow Cyt, ICC/IF, IHC-P, IP, WB

  •  
  • Product image

    Anti-PKC alpha antibody [133] (ab11723)

    Applications: WB

  •  
  • Product image

    Alexa Fluor® 647 Anti-PKC alpha antibody [Y124] (ab204634)

    Applications: ICC/IF

  •  
  • Alexa Fluor® 568 Anti-PKC alpha antibody [Y124] (ab208827)

    Applications: Flow Cyt

  •  
  • Product image

    Alexa Fluor® 488 Anti-PKC alpha antibody [Y124] (ab204633)

    Applications: ICC/IF

Clear all

Recently viewed products

  •  
  • Product image

    Anti-SARS spike glycoprotein antibody [3A2] - Coronavirus (ab272420)

  •  
  • Product image

    Anti-HIP2/LIG antibody [EP1145Y] - BSA and Azide free (ab247338)

  •  
  • Anti-Thrombospondin antibody - BSA and Azide free (ab281867)

Get resources and offers direct to your inbox Sign up
© 2021 Astana Biomed Group LLP. All rights reserved.