Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Tuesday March 09, 2021

Anti-DCI antibody [EPR15375(B)] (ab186752)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR15375(B)] to DCI
Suitable for: IHC-P, ICC/IF, Flow Cyt, WB
Reacts with: Mouse, Rat, Human

Product name

Anti-DCI antibody [EPR15375(B)]
See all DCI primary antibodies
Description

Rabbit monoclonal [EPR15375(B)] to DCI
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Rat Human

See all applications and species data

Immunogen

This information is proprietary to Abcam and/or its suppliers.
Positive control
- Human fetal liver, MCF7, 293T, HeLa, C6, RAW 264.7, PC12 & NIH 3T3 cell lysates; Human kidney and kidney clear cell carcinoma tissues; HeLa and MCF7 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR15375(B)
Isotype

IgG
Research areas

Western blot - Anti-DCI antibody [EPR15375(B)] (ab186752)

All lanes : Anti-DCI antibody [EPR15375(B)] (ab186752) at 1/50000 dilution

Lane 1 : Human fetal liver cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : 293T cell lysate
Lane 4 : HeLa cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 33 kDa
Immunocytochemistry/ Immunofluorescence - Anti-DCI antibody [EPR15375(B)] (ab186752)

Immunofluorescence analysis of HeLa cells labeling DCI with ab186752 at 1/100 dilution. Secondary ab: Goat anti rabbit IgG(Alexa Fluor® 488) at 1/200 dilution. Fixative: 4% paraformaldehyde. Counter stain: Dapi.
Flow Cytometry - Anti-DCI antibody [EPR15375(B)] (ab186752)

Flow cytometric analysis of 2% paraformaldehyde-fixed MCF7 cells labeling DCI with ab186752 at 1/240 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-DCI antibody [EPR15375(B)] (ab186752)

Immunofluorescent analysis of 4% paraformaldehyde-fixed MCF7cells labeling DCI with ab186752 at 1/100 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCI antibody [EPR15375(B)] (ab186752)

Immunohistochemical analysis of paraffin-embedded Human kidney clear cell carcinoma tissue labeling DCI with ab186752 at 1/100 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCI antibody [EPR15375(B)] (ab186752)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling DCI with ab186752 at 1/100 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Western blot - Anti-DCI antibody [EPR15375(B)] (ab186752)

All lanes : Anti-DCI antibody [EPR15375(B)] (ab186752) at 1/10000 dilution

Lane 1 : C6 cell lysate
Lane 2 : RAW 264.7 cell lysate
Lane 3 : PC12 cell lysate
Lane 4 : NIH 3T3 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 33 kDa
Anti-DCI antibody [EPR15375(B)] (ab186752)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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