Anti-DCI antibody [EPR15375(B)] (ab186752)
![Anti-DCI antibody [EPR15375(B)] (ab186752)](https://www.abcam.com/ps/products/186/ab186752/Images/ab186752-220157-anti-dci-antibody-epr15375b-western-blot.jpg "Anti-DCI antibody [EPR15375(B)] (ab186752)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15375(B)] to DCI
- Suitable for: IHC-P, ICC/IF, Flow Cyt, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-DCI antibody [EPR15375(B)]
See all DCI primary antibodies -
Description
Rabbit monoclonal [EPR15375(B)] to DCI -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIHC-P HumanWB RatHuman
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Immunogen
This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal liver, MCF7, 293T, HeLa, C6, RAW 264.7, PC12 & NIH 3T3 cell lysates; Human kidney and kidney clear cell carcinoma tissues; HeLa and MCF7 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15375(B) -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-DCI antibody [EPR15375(B)] (ab186752)All lanes : Anti-DCI antibody [EPR15375(B)] (ab186752) at 1/50000 dilution
Lane 1 : Human fetal liver cell lysate
Lane 2 : MCF7 cell lysate
Lane 3 : 293T cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 33 kDa
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![Immunocytochemistry/ Immunofluorescence - Anti-DCI antibody [EPR15375(B)] (ab186752)](https://www.abcam.com/ps/products/186/ab186752/Images/ab186752-220161-anti-dci-antibody-epr15375b-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-DCI antibody [EPR15375(B)] (ab186752)Immunofluorescence analysis of HeLa cells labeling DCI with ab186752 at 1/100 dilution. Secondary ab: Goat anti rabbit IgG(Alexa Fluor® 488) at 1/200 dilution. Fixative: 4% paraformaldehyde. Counter stain: Dapi.
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![Flow Cytometry - Anti-DCI antibody [EPR15375(B)] (ab186752)](https://www.abcam.com/ps/products/186/ab186752/Images/ab186752-220163-anti-dci-antibody-epr15375b-flow-cytometry.jpg)
Flow Cytometry - Anti-DCI antibody [EPR15375(B)] (ab186752)Flow cytometric analysis of 2% paraformaldehyde-fixed MCF7 cells labeling DCI with ab186752 at 1/240 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
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![Immunocytochemistry/ Immunofluorescence - Anti-DCI antibody [EPR15375(B)] (ab186752)](https://www.abcam.com/ps/products/186/ab186752/Images/ab186752-220162-anti-dci-antibody-epr15375b-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-DCI antibody [EPR15375(B)] (ab186752)Immunofluorescent analysis of 4% paraformaldehyde-fixed MCF7cells labeling DCI with ab186752 at 1/100 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCI antibody [EPR15375(B)] (ab186752)](https://www.abcam.com/ps/products/186/ab186752/Images/ab186752-220160-anti-dci-antibody-epr15375b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCI antibody [EPR15375(B)] (ab186752)Immunohistochemical analysis of paraffin-embedded Human kidney clear cell carcinoma tissue labeling DCI with ab186752 at 1/100 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCI antibody [EPR15375(B)] (ab186752)](https://www.abcam.com/ps/products/186/ab186752/Images/ab186752-220159-anti-dci-antibody-epr15375b-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCI antibody [EPR15375(B)] (ab186752)Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling DCI with ab186752 at 1/100 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Western blot - Anti-DCI antibody [EPR15375(B)] (ab186752)](https://www.abcam.com/ps/products/186/ab186752/Images/ab186752-220158-anti-dci-antibody-epr15375b-western-blot.jpg)
Western blot - Anti-DCI antibody [EPR15375(B)] (ab186752)All lanes : Anti-DCI antibody [EPR15375(B)] (ab186752) at 1/10000 dilution
Lane 1 : C6 cell lysate
Lane 2 : RAW 264.7 cell lysate
Lane 3 : PC12 cell lysate
Lane 4 : NIH 3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 33 kDa
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![Anti-DCI antibody [EPR15375(B)] (ab186752)](https://www.abcam.com/ps/products/186/ab186752/Images/ab186752-8-benefits-of-recombinant-antibodies.png)
Anti-DCI antibody [EPR15375(B)] (ab186752)
