Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Geminin antibody [EPR23754-87] - BSA and Azide free (ab273633)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23754-87] to Geminin - BSA and Azide free
  • Suitable for: Flow Cyt, IP, WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Geminin antibody [EPR23754-87] - BSA and Azide free
    See all Geminin primary antibodies

  • Description

    Rabbit monoclonal [EPR23754-87] to Geminin - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, IP, WB, IHC-Pmore details
    Unsuitable for: ICC/IF

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293, HeLa, PC-12, MEF and NIH/3T3 whole cell lysates; Mouse and rat testis tissue lysate. IHC-P: Human tonsil tissue; Mouse breast carcinoma and testis tissue; Rat testis tissue. Flow Cyt: HEK-293, MEF and HeLa cells. IP: Mouse testis tissue lysate.

  • General notes

    ab273633 is the carrier-free version of ab246509. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab273633 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Geminin antibody [EPR23754-87] - BSA and Azide free (ab273633)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Geminin antibody [EPR23754-87] - BSA and Azide free (ab273633)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling Geminin with ab246509 at 1/100 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human tonsil (PMID: 12107111). The section was incubated with ab246509 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246509).

  • Flow Cytometry - Anti-Geminin antibody [EPR23754-87] - BSA and Azide free (ab273633)
    Flow Cytometry - Anti-Geminin antibody [EPR23754-87] - BSA and Azide free (ab273633)

    Flow cytometric analysis of 80% methanol fixed, 0.1% Tween-20 permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling Geminin with ab246509 at 1/400 dilution (0.1µg) (Right panel) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left panel). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    Cells were pre-treated with 20ug/ml RNaseA for 30 mins to minimize the binding between PI and RNA. Then intracellular stained with ab246509 and PI. Increase expression in S phase and G2/M phase was reported in literature (PMID: 28179914).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246509).

  • Immunoprecipitation - Anti-Geminin antibody [EPR23754-87] - BSA and Azide free (ab273633)
    Immunoprecipitation - Anti-Geminin antibody [EPR23754-87] - BSA and Azide free (ab273633)

    Geminin was immunoprecipitated from 0.35 mg mouse testis tissue lysate with ab246509 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab246509 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse testis tissue lysate 10µg

    Lane 2: ab246509 IP in mouse testis tissue lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab246509 in mouse testis tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246509).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Geminin antibody [EPR23754-87] - BSA and Azide free (ab273633)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Geminin antibody [EPR23754-87] - BSA and Azide free (ab273633)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling Geminin with ab246509 at 1/500 followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse testis (PMID: 22898305, PMID: 28690291). The section was incubated with ab246509 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246509).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Geminin antibody [EPR23754-87] - BSA and Azide free (ab273633)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Geminin antibody [EPR23754-87] - BSA and Azide free (ab273633)

    Immunohistochemical analysis of paraffin-embedded mouse breast carcinoma tissue labeling Geminin with ab246509 at 1/500 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse breast carcinoma (PMID: 12107111). The section was incubated with ab246509 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246509).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Geminin antibody [EPR23754-87] - BSA and Azide free (ab273633)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Geminin antibody [EPR23754-87] - BSA and Azide free (ab273633)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling Geminin with ab246509 at 1/500 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat testis (PMID: 22898305, PMID: 28690291). The section was incubated with ab246509 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246509).

  • Flow Cytometry - Anti-Geminin antibody [EPR23754-87] - BSA and Azide free (ab273633)
    Flow Cytometry - Anti-Geminin antibody [EPR23754-87] - BSA and Azide free (ab273633)

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized MEF (Mouse embryonic fibroblast (immortalized)) cells labelling Geminin with ab246509 at 1/400 dilution (0.1µg) (Right panel) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left panel). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246509).

  • Flow Cytometry - Anti-Geminin antibody [EPR23754-87] - BSA and Azide free (ab273633)
    Flow Cytometry - Anti-Geminin antibody [EPR23754-87] - BSA and Azide free (ab273633)

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized HEK-293 (Human embryonic kidney epithelial cell) cells labelling Geminin with ab246509 at 1/400 dilution (0.1µg) (Right panel) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left panel). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246509).

  • Anti-Geminin antibody [EPR23754-87] - BSA and Azide free (ab273633)
    Anti-Geminin antibody [EPR23754-87] - BSA and Azide free (ab273633)

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