Anti-AMPK alpha 2 antibody (ab231807)
Key features and details
- Rabbit polyclonal to AMPK alpha 2
- Suitable for: WB, IHC-P
- Reacts with: Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-AMPK alpha 2 antibody
See all AMPK alpha 2 primary antibodies -
Description
Rabbit polyclonal to AMPK alpha 2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human, Pig
Predicted to work with: Mouse, Rat, Orangutan
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Immunogen
Recombinant fragment (His-T7-tag) corresponding to Human AMPK alpha 2 aa 250-500. (Expressed in E.coli).
Database link: P54646 -
Positive control
- IHC-P: Human kidney tissue. WB: HeLa, MCF7 and HepG2 cells; Pig skeletal muscle lysate; Recombinant human AMPK alpha 2 protein.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab231807 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPK alpha 2 antibody (ab231807)
Formalin-fixed, paraffin-embedded human kidney tissue stained for AMPK alpha 2 using ab231807 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AMPK alpha 2 antibody (ab231807)Formalin-fixed, paraffin-embedded human kidney tissue stained for AMPK alpha 2 using ab231807 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Western blot - Anti-AMPK alpha 2 antibody (ab231807)Anti-AMPK alpha 2 antibody (ab231807) at 3 µg/ml + Recombinant human AMPK alpha 2 protein
Secondary
HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 62 kDa
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Western blot - Anti-AMPK alpha 2 antibody (ab231807)All lanes : Anti-AMPK alpha 2 antibody (ab231807) at 3 µg/ml
Lane 1 : Pig skeletal muscle lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 3 : MCF7 (human breast adenocarcinoma cell line) cell lysate
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Secondary
All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 62 kDa
