IHC image of Sialoadhesin/CD169 staining in human liver frozen tissue section, fixed in 10% paraformaldehyde (10 min). Staining was performed on a Leica Bond system using the standard protocol F. The section was then incubated with ab18619, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

All lanes : Anti-Sialoadhesin/CD169 antibody [HSn 7D2] (ab18619) at 1 µg/ml

Lane 1 : Human spleen whole tissue lysate
Lane 2 : Human lymph node whole tissue lysate

Lysates/proteins at 40 µg per lane.

Secondary
All lanes : HRP conjugated Goat Anti-Mouse IgG (H+L) at 1/5000 dilution

Performed under reducing conditions.

Exposure time: 20 minutes

This blot was produced using a 3-8% Tris-Acetate gel under the TA buffer system. The gel was run at 100V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab18619 overnight at 4°C. Antibody binding was detected using a Goat anti-mouse antibody conjugated to HRP, and visualised using ECL development solution ab133406.