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Immunology Adaptive Immunity T Cells Non-CD

Anti-BOB1 antibody [EP3300] (ab76010)

Price and availability

304 886 ₸

Availability

Order now and get it on Tuesday March 02, 2021

Anti-BOB1 antibody [EP3300] (ab76010)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP3300] to BOB1
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Human

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Overview

  • Product name

    Anti-BOB1 antibody [EP3300]
    See all BOB1 primary antibodies
  • Description

    Rabbit monoclonal [EP3300] to BOB1
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human BOB1 aa 200-300. The exact sequence is proprietary.

  • Positive control

    • Ramos cell lysate. Human tonsil tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Dissociation constant (KD)

    KD = 1.14 x 10 -10 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EP3300
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Adaptive Immunity
    • B Cells
    • Non-CD
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Developmental Families
    • POU

Images

  • Flow Cytometry - Anti-BOB1 antibody [EP3300] (ab76010)
    Flow Cytometry - Anti-BOB1 antibody [EP3300] (ab76010)

    Flow Cytometry analysis of Jurkat (human acute T cell leukemia) cells labeling BOB1 with unpurified ab76010 at 1/40 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

  • Western blot - Anti-BOB1 antibody [EP3300] (ab76010)
    Western blot - Anti-BOB1 antibody [EP3300] (ab76010)
    Anti-BOB1 antibody [EP3300] (ab76010) at 1/30000 dilution + Ramos cell lysate at 10 µg

    Secondary
    Goat anti-rabbit HRP at 1/1000 dilution

    Predicted band size: 28 kDa
    Observed band size: 35 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BOB1 antibody [EP3300] (ab76010)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BOB1 antibody [EP3300] (ab76010)

    ab76010, at 1/100 dilution, staining BOB1 in human tonsil by immunohistochemistry using paraffin-embedded tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-BOB1 antibody [EP3300] (ab76010)
    OI-RD Scanning - Anti-BOB1 antibody [EP3300] (ab76010)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-BOB1 antibody [EP3300] (ab76010)
    Anti-BOB1 antibody [EP3300] (ab76010)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-BOB1 antibody [EP3300] (ab76010)

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    Applications: IHC-P, Protein Array, WB

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