Antibodies, Proteins, Kits and Reagents for Life Science

Anti-BOB1 antibody [BOB1/2424] - BSA and Azide free (ab237882)

Key features and details

Mouse monoclonal [BOB1/2424] to BOB1 - BSA and Azide free
Suitable for: Protein Array, WB, IHC-P, ICC
Reacts with: Human
Isotype: IgG2b

Product name

Anti-BOB1 antibody [BOB1/2424] - BSA and Azide free
See all BOB1 primary antibodies
Description

Mouse monoclonal [BOB1/2424] to BOB1 - BSA and Azide free
Host species

Mouse
Tested applications

Suitable for: Protein Array, WB, IHC-P, ICCmore details
Species reactivity

Reacts with: Human
Immunogen

Recombinant fragment within Human BOB1 aa 148-255. The exact sequence is proprietary.
Database link: Q16633
Positive control
- WB: Raji cell lysate. IHC-P: Human spleen tissue. ICC: Raji cells
General notes

Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A/G purified
Purification notes

Purified from Bioreactor Concentrate by Protein A/G.
Clonality

Monoclonal
Clone number

BOB1/2424
Isotype

IgG2b
Light chain type

kappa
Research areas

Immunocytochemistry - Anti-BOB1 antibody [BOB1/2424] - BSA and Azide free (ab237882)

Immunofluorescent staining of paraformaldehyde-fixed Raji cells labeling BOB1 with ab237882 at 2µg/mL followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are labeled with RedDot (red).
Western blot - Anti-BOB1 antibody [BOB1/2424] - BSA and Azide free (ab237882)

Anti-BOB1 antibody [BOB1/2424] - BSA and Azide free (ab237882) at 1 µg/ml + Raji (human Burkitt's lymphoma cell line) cell lysate

Predicted band size: 27 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BOB1 antibody [BOB1/2424] - BSA and Azide free (ab237882)

Formalin-fixed, paraffin-embedded human spleen tissue stained for BOB1 using ab237882 at 2 μg/ml in immunohistochemical analysis.
Protein Array - Anti-BOB1 antibody [BOB1/2424] - BSA and Azide free (ab237882)

This data was produced with ab238035, the same antibody in a different formulation with BSA and Azide.
ab238035 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

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