Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-BOB1 antibody [SP92] (ab99891)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP92] to BOB1
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-BOB1 antibody [SP92]
    See all BOB1 primary antibodies

  • Description

    Rabbit monoclonal [SP92] to BOB1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human BOB1 aa 200 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: Q16633

  • Positive control

    • WB: Ramos cell lysate. IHC-P: Human tonsil tissue.

  • General notes

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BOB1 antibody [SP92] (ab99891)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BOB1 antibody [SP92] (ab99891)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling BOB1 with Purified ab99891 at 1/100 dilution (0.96 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Bond™ Epitope Retrieval Solution 1 (pH 6.0) for 20mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody.

    Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BOB1 antibody [SP92] (ab99891)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BOB1 antibody [SP92] (ab99891)
    Staining of Human BOB1 in a Formalin/PFA-fixed paraffin-embedded section of Human Tonsil using ab99891 at a dilution of 1/100.
  • Western blot - Anti-BOB1 antibody [SP92] (ab99891)
    Western blot - Anti-BOB1 antibody [SP92] (ab99891)
    Anti-BOB1 antibody [SP92] (ab99891) at 1/100 dilution + lysate prepared from Ramos cells

    Observed band size: 35 kDa
    why is the actual band size different from the predicted?

  • Anti-BOB1 antibody [SP92] (ab99891)
    Anti-BOB1 antibody [SP92] (ab99891)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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