Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Ly-6A/E antibody [EPR22502-38] (ab219794)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22502-38] to Ly-6A/E
  • Suitable for: WB, Flow Cyt
  • Reacts with: Mouse

Overview

  • Product name

    Anti-Ly-6A/E antibody [EPR22502-38]
    See all Ly-6A/E primary antibodies

  • Description

    Rabbit monoclonal [EPR22502-38] to Ly-6A/E

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Mouse
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse kidney tissue lysate. C2C12 whole cell lysate. Flow Cyt: Concanavalin A treated mouse splenocytes. Mouse bone marrow cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR22502-38

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Ly-6A/E antibody [EPR22502-38] (ab219794)
    Western blot - Anti-Ly-6A/E antibody [EPR22502-38] (ab219794)
    All lanes : Anti-Ly-6A/E antibody [EPR22502-38] (ab219794) at 1/1000 dilution

    Lane 1 : Mouse kidney tissue lysate
    Lane 2 : EL4 (mouse lymphoma T lymphocyte), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 14 kDa


    Exposure time: 48 seconds


    Negative control: EL4 (PMID: 2430016).

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Ly-6A/E antibody [EPR22502-38] (ab219794)
    Western blot - Anti-Ly-6A/E antibody [EPR22502-38] (ab219794)
    Anti-Ly-6A/E antibody [EPR22502-38] (ab219794) at 1/1000 dilution + C2C12 (mouse myoblasts myoblast), whole cell lysate at 20 µg

    Secondary
    VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

    Predicted band size: 14 kDa



    This blot was developed using a higher sensitivity ECL substrate.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Flow Cytometry - Anti-Ly-6A/E antibody [EPR22502-38] (ab219794)
    Flow Cytometry - Anti-Ly-6A/E antibody [EPR22502-38] (ab219794)

    Flow cytometric analysis of mouse splenocytes treated with 2.5μg/ml Concanavalin A (ConA) for 72h labeling Ly-6A/E with ab219794 at 1/500 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.

  • Flow Cytometry - Anti-Ly-6A/E antibody [EPR22502-38] (ab219794)
    Flow Cytometry - Anti-Ly-6A/E antibody [EPR22502-38] (ab219794)

    Flow cytometric analysis of mouse bone marrow cells labeling Ly-6A/E with ab219794 at 1/500 (right panel) compared with a Rabbit monoclonal IgG (ab172730) (left panel). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.

  • Anti-Ly-6A/E antibody [EPR22502-38] (ab219794)
    Anti-Ly-6A/E antibody [EPR22502-38] (ab219794)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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