Anti-Ly-6A/E antibody [EPR22502-38] - BSA and Azide free (ab255803)
![Anti-Ly-6A/E antibody [EPR22502-38] - BSA and Azide free (ab255803)](https://www.abcam.com/ps/products/255/ab255803/Images/ab255803-2-anti-sca1--ly6a-e-antibody-epr22502-38-flowcytometry-bone-marrow-mouse.jpg "Anti-Ly-6A/E antibody [EPR22502-38] - BSA and Azide free (ab255803)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22502-38] to Ly-6A/E - BSA and Azide free
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse
Overview
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Product name
Anti-Ly-6A/E antibody [EPR22502-38] - BSA and Azide free
See all Ly-6A/E primary antibodies -
Description
Rabbit monoclonal [EPR22502-38] to Ly-6A/E - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Mouse
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow Cyt: Concanavalin A treated mouse splenocytes. Mouse bone marrow cells.
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General notes
Ab255803 is the carrier-free version of ab219794. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab255803 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22502-38 -
Isotype
IgG -
Research areas
Images
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Flow Cytometry - Anti-Ly-6A/E antibody [EPR22502-38] - BSA and Azide free (ab255803)
Flow cytometric analysis of mouse bone marrow cells labeling Ly-6A/E with ab219794 at 1/500 (right panel) compared with a Rabbit monoclonal IgG (ab172730) (left panel). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab219794). -
![Flow Cytometry - Anti-Ly-6A/E antibody [EPR22502-38] - BSA and Azide free (ab255803)](https://www.abcam.com/ps/products/255/ab255803/Images/ab255803-1-anti-sca1--ly6a-e-antibody-epr22502-38-flowcytometry-spleen-cona-mouse.jpg)
Flow Cytometry - Anti-Ly-6A/E antibody [EPR22502-38] - BSA and Azide free (ab255803)Flow cytometric analysis of mouse splenocytes treated with 2.5μg/ml Concanavalin A (ConA) for 72h labeling Ly-6A/E with ab219794 at 1/500 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab219794). -
![Anti-Ly-6A/E antibody [EPR22502-38] - BSA and Azide free (ab255803)](https://www.abcam.com/ps/products/255/ab255803/Images/ab255803-3-benefits-of-recombinant-antibodies.png)
Anti-Ly-6A/E antibody [EPR22502-38] - BSA and Azide free (ab255803)
