Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PIM2 antibody [EPR6987] (ab129057)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6987] to PIM2
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-PIM2 antibody [EPR6987]
    See all PIM2 primary antibodies

  • Description

    Rabbit monoclonal [EPR6987] to PIM2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human PIM2 aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control

    • WB: Jurkat, K562, Raji, HL60 and 293T cell lysates, Human heart, Mouse heart, Rat heart, Human kidney, and Mouse kidney tissue lysates; IP: K-562 cell lysate

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-PIM2 antibody [EPR6987] (ab129057)
    Western blot - Anti-PIM2 antibody [EPR6987] (ab129057)
    All lanes : Anti-PIM2 antibody [EPR6987] (ab129057) at 1/10000 dilution (Purified)

    Lane 1 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates
    Lane 2 : Human heart lysates
    Lane 3 : Mouse heart lysates
    Lane 4 : Rat heart lysates
    Lane 5 : Human kidney lysates
    Lane 6 : Mouse kidney lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 34 kDa
    Observed band size: 34-44 kDa
    why is the actual band size different from the predicted?



    The multiple bands are consistent with what has been described in PMID: 7781606 and 24659821 

  • Immunoprecipitation - Anti-PIM2 antibody [EPR6987] (ab129057)
    Immunoprecipitation - Anti-PIM2 antibody [EPR6987] (ab129057)

    ab129057 (purified) at 1:100 dilution (2µg) immunoprecipitating PIM2 in K-562 whole cell lysate.
    Lane 1 (input): K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate 10µg
    Lane 2 (+): ab129057 & K-562 whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab129057 in K-562 whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

  • Western blot - Anti-PIM2 antibody [EPR6987] (ab129057)
    Western blot - Anti-PIM2 antibody [EPR6987] (ab129057)
    All lanes : Anti-PIM2 antibody [EPR6987] (ab129057) at 1/1000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : K562 cell lysate
    Lane 3 : Raji cell lysate
    Lane 4 : HL60 cell lysate
    Lane 5 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 34 kDa

  • OI-RD Scanning - Anti-PIM2 antibody [EPR6987] (ab129057)
    OI-RD Scanning - Anti-PIM2 antibody [EPR6987] (ab129057)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-PIM2 antibody [EPR6987] (ab129057)
    Anti-PIM2 antibody [EPR6987] (ab129057)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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