HRP Anti-MLKL (phospho S345) antibody [EPR9515(2)] (ab208430)
![HRP Anti-MLKL (phospho S345) antibody [EPR9515(2)] (ab208430)](https://www.abcam.com/ps/products/208/ab208430/Images/ab208430-268657-anti-mlkl-phospho-s345-antibody-epr95152-hrp-western-blot.jpg "HRP Anti-MLKL (phospho S345) antibody [EPR9515(2)] (ab208430)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- HRP Rabbit monoclonal [EPR9515(2)] to MLKL (phospho S345)
- Suitable for: Dot blot, WB
- Reacts with: Mouse
- Conjugation: HRP
Overview
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Product name
HRP Anti-MLKL (phospho S345) antibody [EPR9515(2)]
See all MLKL primary antibodies -
Description
HRP Rabbit monoclonal [EPR9515(2)] to MLKL (phospho S345) -
Host species
Rabbit -
Conjugation
HRP -
Specificity
MLKL pS345 is a trigger for necroptosis. It is only detectable in infection/cellular damaged (PMID:29229989) or aging tissue (PMID: 28807105) but not in normal tissues. -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
This information is proprietary to Abcam and/or its suppliers.
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Positive control
- L-929 cells.
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General notes
This antibody was developed through collaboration with the lab of Xiaodong Wang at the National Institute of Biological Sciences, Beijing.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 1% BSA, 30% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9515(2) -
Isotype
IgG -
Research areas
Images
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Western blot - HRP Anti-MLKL (phospho S345) antibody [EPR9515(2)] (ab208430)All lanes : HRP Anti-MLKL (phospho S345) antibody [EPR9515(2)] (ab208430) at 1/4500 dilution
Lane 1 : Untreated L-929 (Mouse connective tissue fibroblast cell line) whole cell lysates
Lane 2 : L-929 (Mouse connective tissue fibroblast cell line) treated with 20 ng/ml TNF alpha,100nM Smac mimetic , and 20mM z-VAD for 8 hours whole cell lysates
Lane 3 : L-929 (Mouse connective tissue fibroblast cell line) treated with 20 ng/ml TNF alpha, 100nM Smac mimetic , and 20mM z-VAD for 8 hours whole cell lysates. Then the membrane was incubated with Alkaline phosphatase.
Lysates/proteins at 15 µg per lane.
Predicted band size: 54 kDa
Observed band size: 54 kDa
Exposure time: 1 minuteBlocking and diluting buffer: 5% NFDM/TBST.
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![Dot Blot - HRP Anti-MLKL (phospho S345) antibody [EPR9515(2)] (ab208430)](https://www.abcam.com/ps/products/208/ab208430/Images/ab208430-268658-anti-mlkl-phospho-s345-antibody-epr95152-hrp-dot-blot.jpg)
Dot Blot - HRP Anti-MLKL (phospho S345) antibody [EPR9515(2)] (ab208430)Dot Blot analysis of
Lane 1: MLKL (phospho S345) phospho peptide and
Lane 2: MLKL Non-phospho peptide labeling MLKL (phospho S345)
with ab208430 at 1/1000 dilution. 5% NFDM/TBST was used as the diluting and blocking buffer. Exposure time: 3 minutes.
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![HRP Anti-MLKL (phospho S345) antibody [EPR9515(2)] (ab208430)](https://www.abcam.com/ps/products/208/ab208430/Images/ab208430-3-benefits-of-recombinant-antibodies.png)
HRP Anti-MLKL (phospho S345) antibody [EPR9515(2)] (ab208430)
