Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MLKL (phospho S358) antibody [EPR9514] - BSA and Azide free (ab208909)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9514] to MLKL (phospho S358) - BSA and Azide free
  • Suitable for: Dot blot, WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-MLKL (phospho S358) antibody [EPR9514] - BSA and Azide free
    See all MLKL primary antibodies

  • Description

    Rabbit monoclonal [EPR9514] to MLKL (phospho S358) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    Stimulation may be required to allow detection of the phosphorylated protein. Please see images below for recommended treatment conditions and positive controls.

  • Tested applications

    Suitable for: Dot blot, WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
    (Peptide available as ab206929)

  • Positive control

    • HT-29 cell lysate treated with TNF alpha+ Smac mimetic+ z-VAD HT-29 cells were treated with the indicated stimuli for 8 hr and then harvested. The ?nal concentrations of 20 ng/ml TNF-a, 100 nM Smac mimetic, and 20 mM z-VAD were used to induce necrosis

  • General notes

    ab208909 is the carrier-free version of ab187091.

    This antibody was developed through collaboration with the lab of Xiaodong Wang at the National Institute of Biological Sciences, Beijing.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Dot Blot - Anti-MLKL (phospho S358) antibody [EPR9514] - BSA and Azide free (ab208909)
    Dot Blot - Anti-MLKL (phospho S358) antibody [EPR9514] - BSA and Azide free (ab208909)

    Dot blot analysis of MLKL peptides using ab187091 at 1/1000 dilution followed by Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated secondary antibody at 1/1000 dilution. Blocking and diluting buffer was 5% NFDM/TBST, exposure time 3 minuts.

     

    Lane 1: MLKL (pT357) phospho peptide

    Lane 2: MLKL (pS358) phospho peptide

    Lane 3: MLKL (pT357/pS358) phospho peptide

    Lane 4: MLKL non-phospho peptide 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab187091).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MLKL (phospho S358) antibody [EPR9514] - BSA and Azide free (ab208909)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MLKL (phospho S358) antibody [EPR9514] - BSA and Azide free (ab208909)

    ab187091 at 1:250 staining MLKL (phospho S358) in Human melanoma tissue by immunohistochemistry (FFPE).

     

    Antigen retrival required on FFPE tissue:  HIER using 10mM Citrate buffer pH 6.0, see recommended HIER protocol

     

    For additional IHC guideline, please see IHC resource page

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab187091).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MLKL (phospho S358) antibody [EPR9514] - BSA and Azide free (ab208909)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MLKL (phospho S358) antibody [EPR9514] - BSA and Azide free (ab208909)

    This IHC data was generated using the same anti-MLKL (phospho S358) in a different buffer formulation (cat# ab187091).

    ab187091 at 1:250 staining MLKL (phospho S358) in Human skin tissue by immunohistochemistry (FFPE).

     

    Antigen retrival required on FFPE tissue: HIER using 10mM Citrate buffer pH 6.0, see recommended HIER protocol

    For additional IHC guideline, please see IHC resource page

  • Anti-MLKL (phospho S358) antibody [EPR9514] - BSA and Azide free (ab208909)
    Anti-MLKL (phospho S358) antibody [EPR9514] - BSA and Azide free (ab208909)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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