Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-DCTN3 antibody [EPR5097] - BSA and Azide free (ab232440)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5097] to DCTN3 - BSA and Azide free
  • Suitable for: IHC-P, WB, ICC/IF
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-DCTN3 antibody [EPR5097] - BSA and Azide free
    See all DCTN3 primary antibodies

  • Description

    Rabbit monoclonal [EPR5097] to DCTN3 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human pancreas tissue.

  • General notes

    Ab232440 is the carrier-free version of ab124674. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab232440 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-DCTN3 antibody [EPR5097] - BSA and Azide free (ab232440)
    Immunocytochemistry/ Immunofluorescence - Anti-DCTN3 antibody [EPR5097] - BSA and Azide free (ab232440)

    Immunofluorescence staining of BxPC-3 cells with purified ab124674 at a working dilution of 1 in 100, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti rabbit (ab150077), used at a dilution of 1 in 500. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative control is shown in bottom right hand panel - for the negative control, purified ab124674 was used at a dilution of 1/200 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab15120) at a dilution of 1/500.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124674).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN3 antibody [EPR5097] - BSA and Azide free (ab232440)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN3 antibody [EPR5097] - BSA and Azide free (ab232440)

    Immunohistochemical staining of DCTN3 in paraffin embedded human breast tissue by unpurified ab124674 at 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124674).

    Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

  • OI-RD Scanning - Anti-DCTN3 antibody [EPR5097] - BSA and Azide free (ab232440)
    OI-RD Scanning - Anti-DCTN3 antibody [EPR5097] - BSA and Azide free (ab232440)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124674).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN3 antibody [EPR5097] - BSA and Azide free (ab232440)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN3 antibody [EPR5097] - BSA and Azide free (ab232440)

    Immunohistochemical staining of paraffin embedded human pancreas with purified ab124674 at a working dilution of 1 in 100. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab124674).

  • Anti-DCTN3 antibody [EPR5097] - BSA and Azide free (ab232440)
    Anti-DCTN3 antibody [EPR5097] - BSA and Azide free (ab232440)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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