Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-DCTN3 antibody [EPR5097] (ab124674)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5097] to DCTN3
  • Suitable for: ICC/IF, WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-DCTN3 antibody [EPR5097]
    See all DCTN3 primary antibodies

  • Description

    Rabbit monoclonal [EPR5097] to DCTN3

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human DCTN3 aa 100-200. The exact sequence is proprietary.
    Database link: O75935

  • Positive control

    • 293T, HeLa, BxPC 3, SH-SY5Y and mouse and rat muscle lysates; Human breast tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-DCTN3 antibody [EPR5097] (ab124674)
    Western blot - Anti-DCTN3 antibody [EPR5097] (ab124674)
    All lanes : Anti-DCTN3 antibody [EPR5097] (ab124674) at 1/10000 dilution (purified)

    Lane 1 : 293T cell lysate
    Lane 2 : SH-SY5Y cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 21 kDa
    Observed band size: 21 kDa



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN3 antibody [EPR5097] (ab124674)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN3 antibody [EPR5097] (ab124674)

    Immunohistochemical staining of DCTN3 in paraffin embedded human breast tissue by unpurified ab124674 at 1/100 dilution.

    Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN3 antibody [EPR5097] (ab124674)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN3 antibody [EPR5097] (ab124674)

    Immunohistochemical staining of paraffin embedded human pancreas with purified ab124674 at a working dilution of 1 in 100. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Immunocytochemistry/ Immunofluorescence - Anti-DCTN3 antibody [EPR5097] (ab124674)
    Immunocytochemistry/ Immunofluorescence - Anti-DCTN3 antibody [EPR5097] (ab124674)

    Immunofluorescence staining of BxPC-3 cells with purified ab124674 at a working dilution of 1 in 100, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti rabbit (ab150077), used at a dilution of 1 in 500. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative control is shown in bottom right hand panel - for the negative control, purified ab124674 was used at a dilution of 1/200 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab15120) at a dilution of 1/500.

  • Western blot - Anti-DCTN3 antibody [EPR5097] (ab124674)
    Western blot - Anti-DCTN3 antibody [EPR5097] (ab124674)
    Anti-DCTN3 antibody [EPR5097] (ab124674) at 1/10000 dilution (purified) + Rat muscle at 20 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 21 kDa
    Observed band size: 21 kDa



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-DCTN3 antibody [EPR5097] (ab124674)
    Western blot - Anti-DCTN3 antibody [EPR5097] (ab124674)
    Anti-DCTN3 antibody [EPR5097] (ab124674) at 1/2000 dilution (purified) + Mouse muscle at 10 µg

    Secondary
    HRP goat anti-rabbit (H+L) at 1/1000 dilution

    Predicted band size: 21 kDa
    Observed band size: 21 kDa



    Blocking buffer: 5% NFDM/TBST

    Dilution buffer: 5% NFDM/TBST

  • Western blot - Anti-DCTN3 antibody [EPR5097] (ab124674)
    Western blot - Anti-DCTN3 antibody [EPR5097] (ab124674)
    All lanes : Anti-DCTN3 antibody [EPR5097] (ab124674) at 1/1000 dilution (unpurified)

    Lane 1 : 293T cell lysates
    Lane 2 : Mouse muscle lysates
    Lane 3 : HeLa cell lysates
    Lane 4 : BxPC 3 cell lysates
    Lane 5 : SH-SY5Y cell lysates
    Lane 6 : Rat muscle lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    Lanes 1-3 & 5-6 : Goat anti-Rabbit HRP at 1/2000 dilution


    Predicted band size: 21 kDa

  • OI-RD Scanning - Anti-DCTN3 antibody [EPR5097] (ab124674)
    OI-RD Scanning - Anti-DCTN3 antibody [EPR5097] (ab124674)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-DCTN3 antibody [EPR5097] (ab124674)
    Anti-DCTN3 antibody [EPR5097] (ab124674)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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