Human Cathepsin C ELISA Kit (ab277434)
Key features and details
- Sensitivity: 2 ng/ml
- Range: 2.048 ng/ml - 500 ng/ml
- Sample type: Cell culture supernatant, Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Cathepsin C ELISA Kit
See all Cathepsin C kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Hep Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
2 ng/ml -
Range
2.048 ng/ml - 500 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 80.54 73% - 95% Plasma 71.93 68% - 80% Cell culture media 135.7 124% - 150% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Human Cathepsin C ELISA Kit (ab277434) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Cathepsin C in serum, plasma (collect plasma using citrate and heparin as an anticoagulant. EDTA is not recommended as anticoagulants for use in this assay) and cell culture supernatants.
This assay employs an antibody specific for Human Cathepsin C coated on a 96-well plate. Standards and samples are pipetted into the wells and Human Cathepsin C present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human Cathepsin C antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human Cathepsin C bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 100X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent 1 x 15ml Biotinylated Anti-Human Cathepsin C Antibody 2 vials Anti-Human Cathepsin C coated Microplate 1 unit Human Cathepsin C standard protein (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Thiol protease. Has dipeptidylpeptidase activity. Active against a broad range of dipeptide substrates composed of both polar and hydrophobic amino acids. Proline cannot occupy the P1 position and arginine cannot occupy the P2 position of the substrate. Can act as both an exopeptidase and endopeptidase. Activates serine proteases such as elastase, cathepsin G and granzymes A and B. Can also activate neuraminidase and factor XIII. -
Tissue specificity
Ubiquitous. Highly expressed in lung, kidney and placenta. Detected at intermediate levels in colon, small intestine, spleen and pancreas. -
Involvement in disease
Papillon-Lefevre syndrome
Haim-Munk syndrome
Periodontititis, aggressive, 1 -
Sequence similarities
Belongs to the peptidase C1 family. -
Post-translational
modificationsN-glycosylated. While glycosylation at Asn-53, Asn-119 and Asn-276 is mediated by STT3A-containing complexes, glycosylation at Asn-29 is mediated STT3B-containing complexes.
In approximately 50% of the complexes the exclusion domain is cleaved at position 58 or 61. The two parts of the exclusion domain are held together by a disulfide bond. -
Cellular localization
Lysosome. - Information by UniProt
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Alternative names
- AI047818
- CATC
- CATC_HUMAN
see all -
Database links
- Entrez Gene: 1075 Human
- Omim: 602365 Human
- SwissProt: P53634 Human
- Unigene: 128065 Human
Images
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Example data
These standard curves are for demonstration only. A standard curve must be run with each assay.