Anti-MLKL (phospho Y222) antibody [EPR19816] (ab206336)
![Anti-MLKL (phospho Y222) antibody [EPR19816] (ab206336)](https://www.abcam.com/ps/products/206/ab206336/Images/ab206336-276240-anti-mlkl-phospho-y222-antibody-epr19816-western-blot.jpg "Anti-MLKL (phospho Y222) antibody [EPR19816] (ab206336)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19816] to MLKL (phospho Y222)
- Suitable for: WB
- Reacts with: Human
Overview
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Product name
Anti-MLKL (phospho Y222) antibody [EPR19816]
See all MLKL primary antibodies -
Description
Rabbit monoclonal [EPR19816] to MLKL (phospho Y222) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK-293T transfected with NPM (nucleophosmin)-ALK (anaplastic lymphoma kinase) and MLKL-WT expression vector, whole cell lysate; HEK-293T transfected with NPM (nucleophosmin)-ALK (anaplastic lymphoma kinase) and MLKL-Y23F mutant expression vector, whole cell lysate; HEK-293T transfected with NPM (nucleophosmin)-ALK (anaplastic lymphoma kinase) and MLKL-Y218F mutant expression vector, whole cell lysate
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19816 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-MLKL (phospho Y222) antibody [EPR19816] (ab206336)All lanes : Anti-MLKL (phospho Y222) antibody [EPR19816] (ab206336) at 1/1000 dilution
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney) transfected with MLKL-WT expression vector, whole cell lysate at 20 µg
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney) transfected with NPM (nucleophosmin)-ALK (anaplastic lymphoma kinase) and MLKL-WT expression vector, whole cell lysate at 4 µg
Lane 3 : HEK-293T (Human epithelial cell line from embryonic kidney) transfected with NPM (nucleophosmin)-ALK (anaplastic lymphoma kinase) and MLKL-Y23F mutant expression vector, whole cell lysate at 20 µg
Lane 4 : HEK-293T (Human epithelial cell line from embryonic kidney) transfected with NPM (nucleophosmin)-ALK (anaplastic lymphoma kinase) and MLKL-Y218F mutant expression vector, whole cell lysate at 20 µg
Lane 5 : HEK-293T (Human epithelial cell line from embryonic kidney) transfected with NPM (nucleophosmin)-ALK (anaplastic lymphoma kinase) and MLKL-Y222F mutant expression vector, whole cell lysate at 20 µg
Lane 6 : HEK-293T (Human epithelial cell line from embryonic kidney) transfected with NPM (nucleophosmin)-ALK (anaplastic lymphoma kinase) and MLKL-Y218/222F mutant expression vector, whole cell lysate at 8 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 54 kDa
Observed band size: 54 kDa
Exposure time: 1 minuteBlocking/Dilution buffer: 5% NFDM/TBST.
Lysates were kindly provided by our collaborator Dr. Xinhua Feng, Zhejiang University.
The molecular weight of NPM-ALK is around 80 kDa (lane 2) [PMID: 11090048].
This antibody can potentially cross react with other kinases.
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![Anti-MLKL (phospho Y222) antibody [EPR19816] (ab206336)](https://www.abcam.com/ps/products/206/ab206336/Images/ab206336-4-benefits-of-recombinant-antibodies.png)
Anti-MLKL (phospho Y222) antibody [EPR19816] (ab206336)
