Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MLKL (phospho S345) antibody [EPR9515(2)] - BSA and Azide free (ab208910)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9515(2)] to MLKL (phospho S345) - BSA and Azide free
  • Suitable for: WB, IP, Dot blot
  • Reacts with: Mouse

Overview

  • Product name

    Anti-MLKL (phospho S345) antibody [EPR9515(2)] - BSA and Azide free
    See all MLKL primary antibodies

  • Description

    Rabbit monoclonal [EPR9515(2)] to MLKL (phospho S345) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    MLKL pS345 is a trigger for necroptosis. It is only detectable in infection/cellular damaged (PMID:29229989) or aging tissue (PMID: 28807105) but not in normal tissues.

  • Tested Applications & Species

    Application Species
    IP
    Mouse
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: L929 whole cell lysate treated with 20 ng/ml TNF alpha, 100 nM Smac mimetic, and 20 mM z-VAD for 8h and then harvested. IP: L929 whole cell lysate treated with TNF alpha+ Smac mimetic + z-VAD compound for 8h.

  • General notes

    ab208910 is the carrier-free version of ab196436 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab208910 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This antibody was developed through collaboration with the lab of Xiaodong Wang at the National Institute of Biological Sciences, Beijing.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-MLKL (phospho S345) antibody [EPR9515(2)] - BSA and Azide free (ab208910)
    Immunoprecipitation - Anti-MLKL (phospho S345) antibody [EPR9515(2)] - BSA and Azide free (ab208910)

    MLKL (phospho S345) was immunoprecipitated from 1mg of L-929 (Mouse connective tissue fibroblast cells) whole cell lysate treated with 20 ng/ml TNF alpha + 100 nM Smac mimetic + 20 µM z-VAD compound for 8h using ab196436 at 1/150 dilution. Western blot was performed from the immunoprecipitate using ab196436 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: L-929 whole cell lysate treated with 20 ng/ml TNF alpha+ 100 nM Smac mimetic+ 20 µM z-VAD compound for 8h;10 µg (Input).
    Lane 2: ab196436 IP in L-929 whole cell lysate treated with 20 ng/ml TNF alpha+ 100 nM Smac mimetic+ 20 µM z-VAD compound for 8h.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab196436 in L-929 whole cell lysate treated with 20 ng/ml TNF alpha+ 100 nM Smac mimetic+ 20 µM z-VAD compound for 8h.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab196436).

  • Dot Blot - Anti-MLKL (phospho S345) antibody [EPR9515(2)] - BSA and Azide free (ab208910)
    Dot Blot - Anti-MLKL (phospho S345) antibody [EPR9515(2)] - BSA and Azide free (ab208910)

    This Dot Blot data was generated using the same anti-MLKL (phospho S345) antibody clone, EPR9515(2), in a different buffer formulation (cat# ab196436).

    Dot blot analysis of MLKL (phospho S345) peptide (Lane 1), and non-phospho peptide (Lane 2), labeled using ab196436 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Anti-MLKL (phospho S345) antibody [EPR9515(2)] - BSA and Azide free (ab208910)
    Anti-MLKL (phospho S345) antibody [EPR9515(2)] - BSA and Azide free (ab208910)

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