Anti-PASK antibody (ab114988)
Key features and details
- Rabbit polyclonal to PASK
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PASK antibody
See all PASK primary antibodies -
Description
Rabbit polyclonal to PASK -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide, corresponding to a region within amino acids 425-475 of Human PASK (NP_055963.2).
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Positive control
- HeLa, 293T and Jurkat whole cell lysate (ab7899).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab114988 was affinity purified using an epitope specific to PASK immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PASK antibody (ab114988)All lanes : Anti-PASK antibody (ab114988) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : 293T whole cell lysate at 50 µg
Lane 4 : Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 143 kDa
Exposure time: 3 minutes
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Immunoprecipitation - Anti-PASK antibody (ab114988)Detection of PASK by Western Blot of Immunprecipitate.
ab114988 at 0.4µg/ml staining PASK in HeLa whole cell lysate immunoprecipitated using ab114988 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 3 mminutes.
All lanes : Anti-PASK antibody (ab114988) at 0.4 µg/ml
Lane 1 : HeLa cell lysate, IP with ab114988 at 6 µg per mg lysate
Lane 2 : HeLa cell lysate, IP with alternative PASK antibody
Lane 3 : HeLa cell lysate, IP with control IgG
Lysates/proteins at 200 µg per lane.
Developed using the ECL technique.
Exposure time: 3 minutes
