Anti-PASK antibody (ab115164)
Key features and details
- Rabbit polyclonal to PASK
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PASK antibody
See all PASK primary antibodies -
Description
Rabbit polyclonal to PASK -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human -
Immunogen
Synthetic peptide, corresponding to a region within amino acids 1273 -1323 of Human PASK (Q96RG2).
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Positive control
- HeLa, 293T and Jurkat cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-PASK antibody (ab115164) at 0.4 µg/ml
Lane 1 : HeLa Cell lysate at 50 µg
Lane 2 : HeLa Cell lysate at 15 µg
Lane 3 : 293T Cell lysate at 50 µg
Lane 4 : Jurkat Cell lysate at 50 µg
Predicted band size: 143 kDa
Exposure time: 10 seconds
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Immunoprecipitation of PASK (6 µg of antibody per mg of HeLa cell lysate; 20% of the IP loaded). Lane 1- Immunoprecipitation with an anti-PASK antibody that recognizes an upstream epitope; Lane 2-Immunoprecipitation with anti-PASK antibody (ab115164); Lane 3- Immunoprecipitation with IgG control. Anti-PASK antibody (ab115164) used at 1 µg/ml for western blot. Detection: Chemiluminescence with exposure time of 10 sec.