Anti-NAK/TBK1 (phospho S172) antibody [EPR2867(2)] - BSA and Azide free (ab186469)
![Anti-NAK/TBK1 (phospho S172) antibody [EPR2867(2)] - BSA and Azide free (ab186469)](https://www.abcam.com/ps/products/186/ab186469/Images/ab186469-327582-anti-nak-tbk1-phospho-s172-antibody-epr28672-dot-blot.jpg "Anti-NAK/TBK1 (phospho S172) antibody [EPR2867(2)] - BSA and Azide free (ab186469)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2867(2)] to NAK/TBK1 (phospho S172) - BSA and Azide free
- Suitable for: Dot blot, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-NAK/TBK1 (phospho S172) antibody [EPR2867(2)] - BSA and Azide free
See all NAK/TBK1 primary antibodies -
Description
Rabbit monoclonal [EPR2867(2)] to NAK/TBK1 (phospho S172) - BSA and Azide free -
Host species
Rabbit -
Specificity
This antibody only detects NAK/TBK1 phosphorylated at serine 172. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
(Peptide available asab206124) -
Positive control
- WB: NIH/3T3 treated with 10mM pervanadate for 30 minutes, whole cell lysate.
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General notes
Ab186469 is the carrier-free version of ab109272. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab186469 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2867(2) -
Isotype
IgG -
Research areas
Images
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Dot Blot - Anti-NAK/TBK1 (phospho S172) antibody [EPR2867(2)] - BSA and Azide free (ab186469)
Dot blot analysis of NAK/TBK1 (phospho S172) phospho peptide (Lane 1) and NAK/TBK1 non-phospho peptide (Lane 2) labelling NAK/TBK1 (phospho S172) phospho peptide with ab109272 at a dilution of 1:1000 dilution (0.627μg/ml). A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1:20,000 dilution.
Blocking buffer: 5% NFDM/TBST.
Dilution buffer: 5% NFDM /TBST .This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109272).
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![Western blot - Anti-NAK/TBK1 (phospho S172) antibody [EPR2867(2)] - BSA and Azide free (ab186469)](https://www.abcam.com/ps/products/186/ab186469/Images/ab186469-311795-anti-nak-tbk1-phospho-s172-antibody-epr28672-western-blot.jpg)
Western blot - Anti-NAK/TBK1 (phospho S172) antibody [EPR2867(2)] - BSA and Azide free (ab186469)All lanes : Anti-NAK/TBK1 (phospho S172) antibody [EPR2867(2)] (ab109272) at 1/5000 dilution
Lane 1 : Untreated NIH/3T3 (mouse embryo) whole cell lysate
Lane 2 : NIH/3T3 (mouse embryo) treated with 10mM pervanadate for 30 minutes whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 84 kDaBlocking buffer and diluting buffer: 5% NFDM /TBST
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109272).
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![Anti-NAK/TBK1 (phospho S172) antibody [EPR2867(2)] - BSA and Azide free (ab186469)](https://www.abcam.com/ps/products/186/ab186469/Images/ab186469-3-benefits-of-recombinant-antibodies.png)
Anti-NAK/TBK1 (phospho S172) antibody [EPR2867(2)] - BSA and Azide free (ab186469)
