Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NAK/TBK1 (phospho S172) antibody [EPR2867(2)] - BSA and Azide free (ab186469)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2867(2)] to NAK/TBK1 (phospho S172) - BSA and Azide free
  • Suitable for: Dot blot, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-NAK/TBK1 (phospho S172) antibody [EPR2867(2)] - BSA and Azide free
    See all NAK/TBK1 primary antibodies

  • Description

    Rabbit monoclonal [EPR2867(2)] to NAK/TBK1 (phospho S172) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody only detects NAK/TBK1 phosphorylated at serine 172.

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
    (Peptide available as ab206124)

  • Positive control

    • WB: NIH/3T3 treated with 10mM pervanadate for 30 minutes, whole cell lysate.

  • General notes

    Ab186469 is the carrier-free version of ab109272. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab186469 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Dot Blot - Anti-NAK/TBK1 (phospho S172) antibody [EPR2867(2)] - BSA and Azide free (ab186469)
    Dot Blot - Anti-NAK/TBK1 (phospho S172) antibody [EPR2867(2)] - BSA and Azide free (ab186469)

    Dot blot analysis of NAK/TBK1 (phospho S172) phospho peptide (Lane 1) and NAK/TBK1 non-phospho peptide (Lane 2) labelling NAK/TBK1 (phospho S172) phospho peptide with ab109272 at a dilution of 1:1000 dilution (0.627μg/ml). A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1:20,000 dilution.

    Blocking buffer: 5% NFDM/TBST.
    Dilution buffer: 5% NFDM /TBST .

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109272).

  • Western blot - Anti-NAK/TBK1 (phospho S172) antibody [EPR2867(2)] - BSA and Azide free (ab186469)
    Western blot - Anti-NAK/TBK1 (phospho S172) antibody [EPR2867(2)] - BSA and Azide free (ab186469)
    All lanes : Anti-NAK/TBK1 (phospho S172) antibody [EPR2867(2)] (ab109272) at 1/5000 dilution

    Lane 1 : Untreated NIH/3T3 (mouse embryo) whole cell lysate
    Lane 2 : NIH/3T3 (mouse embryo) treated with 10mM pervanadate for 30 minutes whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

    Predicted band size: 84 kDa



    Blocking buffer and diluting buffer: 5% NFDM /TBST

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109272).

  • Anti-NAK/TBK1 (phospho S172) antibody [EPR2867(2)] - BSA and Azide free (ab186469)
    Anti-NAK/TBK1 (phospho S172) antibody [EPR2867(2)] - BSA and Azide free (ab186469)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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