Recombinant human AMPK alpha 2 + beta 1 + gamma 2 protein (ab185258)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% SDS-PAGE
- Active: Yes
- Tags: His tag C-Terminus, proprietary tag N-Terminus
- Suitable for: WB, Functional Studies, SDS-PAGE
-
Product name
Recombinant human AMPK alpha 2 + beta 1 + gamma 2 protein -
Biological activity
The specific activity of ab185258 was determined to be 350 nmol/min/mg.
-
Purity
> 90 % SDS-PAGE.
Assessed by densitometry. -
Expression system
Baculovirus infected Sf9 cells -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
-
Species
Human -
Tags
His tag C-Terminus , proprietary tag N-Terminus -
Additional sequence information
Recombinant full length AMPK alpha 2 (aa 1-552; UniProt P54646) + AMPK beta 1 (aa 1-270; UniProt Q9Y478) + AMPK gamma 2 Isoform C (aa 1-525; UniProt Q9UGJ0-3). MWt 92, 65, and 105 kDa respectively.
-
Preparation and Storage
-
Applications
Western blot
Functional Studies
SDS-PAGE
-
Form
Liquid -
Additional notes
AMPK alpha 2 (amino acids: 1-552; UniProt P54646)
MAEKQKHDGRVKIGHYVLGDTLGVGTFGKVKIGEHQLTGHKVAVKILNRQKIRSLDVVGKIKREIQNLKLFRHPHIIKLYQVISTPTDFFMVMEYVSGGELFDYICKHGRVEEMEARRLFQQILSAVDYCHRHMVVHRDLKPENVLLDAHMNAKIADFGLSNMMSDGEFLRTSCGSPNYAAPEVISGRLYAGPEVDIWSCGVILYALLCGTLPFDDEHVPTLFKKIRGGVFYIPEYLNRSVATLLMHMLQVDPLKRATIKDIREHEWFKQDLPSYLFPEDPSYDANVIDDEAVKEVCEKFECTESEVMNSLYSGDPQDQLAVAYHLIIDNRRIMNQASEFYLASSPPSGSFMDDSAMHIPPGLKPHPERMPPLIADSPKARCPLDALNTTKPKSLAVKKAKWHLGIRSQSKPYDIMAEVYRAMKQLDFEWKVVNAYHLRVRRKNPVTGNYVKMSLQLYLVDNRSYLLDFKSIDDEVVEQRSGSSTPQRSCSAAGLHRPRSSFDSTTAESHSLSGSLTGSLTGSTLSSVSPRLGSHTMDFFEMCASLITTLAR
AMPK beta 1 (amino acids 1-270; UniProt Q9Y478)
MGNTSSERAALERHGGHKTPRRDSSGGTKDGDRPKILMDSPEDADLFHSEEIKAPEKEEFLAWQHDLEVNDKAPAQARPTVFRWTGGGKEVYLSGSFNNWSKLPLTRSHNNFVAILDLPEGEHQYKFFVDGQWTHDPSEPIVTSQLGTVNNIIQVKKTDFEVFDALMVDSQKCSDVSELSSSPPGPYHQEPYVCKPEERFRAPPILPPHLLQVILNKDTGISCDPALLPEPNHVMLNHLYALSIKDGVMVLSATHRYKKKYVTTLLYKPI
AMPK gamma 2 Isoform C (amino acids 1-525; UniProt Q9UGJ0-3)
MPLLDGDLEGSGKHSSRKVDSPFGPGSPSKGFFSRGPQPRPSSPMSAPVRPKTSPGSPKTVFPFSYQESPPRSPRRMSFSGIFRSSSKESSPNSNPATSPGGIRFFSRSRKTSGLSSSPSTPTQVTKQHTFPLESYKHEPERLENRIYASSSPPDTGQRFCPSSFQSPTRPPLASPTHYAPSKAAALAAALGPAEAGMLEKLEFEDEAVEDSESGVYMRFMRSHKCYDIVPTSSKLVVFDTTLQVKKAFFALVANGVRAAPLWESKKQSFVGMLTITDFINILHRYYKSPMVQIYELEEHKIETWRELYLQETFKPLVNISPDASLFDAVYSLIKNKIHRLPVIDPISGNALYILTHKRILKFLQLFMSDMPKPAFMKQNLDELGIGTYHNIAFIHPDTPIIKALNIFVERRISALPVVDESGKVVDIYSKFDVINLAAEKTYNNLDITVTQALQHRSQYFEGVVKCNKLEILETIVDRIVRAEVHRLVVVNEADSIVGIISLSDILQALILTPAGAKQKETETEab204881 (Acetyl Coenzyme A Carboxylase peptide) can be utilized as a substrate for assessing kinase activity
-
Concentration information loading...
Images
-
Functional Studies - Recombinant human AMPK alpha 2 + beta 1 + gamma 2 protein (ab185258)The specific activity of AMPK alpha 2 + beta 1 + gamma 2 (ab185258) was determined to be 297 nmol/min/mg as per activity assay protocol
-
SDS-PAGE - Recombinant human AMPK alpha 2 + beta 1 + gamma 2 protein (ab185258)SDS PAGE analysis of ab185258 -
SDS-PAGE - Recombinant human AMPK alpha 2 + beta 1 + gamma 2 protein (ab185258)SDS PAGE analysis of ab185258 -
SDS-PAGE - Recombinant human AMPK alpha 2 + beta 1 + gamma 2 protein (ab185258)SDS-PAGE analysis of ab185258.
-
Functional Studies - Recombinant human AMPK alpha 2 + beta 1 + gamma 2 protein (ab185258)Kinase Assay showing the specific activity of ab185258 as 350 nmol/min/mg.