Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NAK/TBK1 antibody [EP611Y] (ab40676)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP611Y] to NAK/TBK1
  • Suitable for: ICC/IF, WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-NAK/TBK1 antibody [EP611Y]
    See all NAK/TBK1 primary antibodies

  • Description

    Rabbit monoclonal [EP611Y] to NAK/TBK1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human NAK/TBK1 aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control

    • WB: HeLa membrane extract lysate (ab29547), HepG2, SH-SY5Y, C6, HAP1 and NIH/3T3 cell lysate IHC-P: Human hepatocellular carcinoma ICC/IF: MCF7 cells

  • General notes

    Anti-NAK/TBK1 antibody [EP611Y] (ab40676) may not be suitable for IHC with mouse or rat samples.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-NAK/TBK1 antibody [EP611Y] (ab40676)
    Western blot - Anti-NAK/TBK1 antibody [EP611Y] (ab40676)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: NAK knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: HepG2 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab40676 observed at 90 kDa. Red - loading control, ab8245, observed at 37 kDa.
    ab40676 was shown to specifically react with NAK when NAK knockout samples were used. Wild-type and NAK knockout samples were subjected to SDS-PAGE. ab40676 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10,000  respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 h at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAK/TBK1 antibody [EP611Y] (ab40676)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAK/TBK1 antibody [EP611Y] (ab40676)

    Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue sections labeling NAK/TBK1 with purified ab40676 at a dilution of 1/100 (11.5 μg/ml). ab97051 Goat Anti-Rabbit IgG H&L (HRP) at 1/500 was used as the secondary anitbody. Sections were counterstained with hematoxylin. Antigen retrieval was heat mediated using EDTA Buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset. 

  • Western blot - Anti-NAK/TBK1 antibody [EP611Y] (ab40676)
    Western blot - Anti-NAK/TBK1 antibody [EP611Y] (ab40676)
    All lanes : Anti-NAK/TBK1 antibody [EP611Y] (ab40676) at 1/5000 dilution (purified)

    Lane 1 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 2 : SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate
    Lane 3 : C6 (Rat glial tumor cell line) whole cell lysate
    Lane 4 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 84 kDa
    Observed band size: 84 kDa



    Blocking/Diluting buffer 5% NFDM/TBST
  • Immunocytochemistry/ Immunofluorescence - Anti-NAK/TBK1 antibody [EP611Y] (ab40676)
    Immunocytochemistry/ Immunofluorescence - Anti-NAK/TBK1 antibody [EP611Y] (ab40676)

    Immunocytochemistry/Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma) cells labeling NAK/TBK1 with purified ab40676 at 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluo®488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Cells were counter-stained with ab195889 Anti-Alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor®594) at 1/200. DAPI (blue) was used as a nuclear counterstain. Secondary Only Control: PBS was used instead of the primary antibody as the negative control.

  • Anti-NAK/TBK1 antibody [EP611Y] (ab40676)
    Anti-NAK/TBK1 antibody [EP611Y] (ab40676)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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