Antibodies, Proteins, Kits and Reagents for Life Science

Anti-beta Catenin antibody [BLR086G] (ab265591)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [BLR086G] to beta Catenin
Suitable for: WB, IP, ICC, IHC-P
Reacts with: Mouse, Human

Product name

Anti-beta Catenin antibody [BLR086G]
See all beta Catenin primary antibodies
Description

Rabbit monoclonal [BLR086G] to beta Catenin
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC	Human
IHC-P	Human
IP	Human
WB	Mouse Human

See all applications and species data

Immunogen

Synthetic peptide within Human beta Catenin aa 725-775. The exact sequence is proprietary. NP_001895.1
Database link: P35222
Positive control
- IHC-P: Human colon carcinoma tissue. Human plasmacytoma. ICC: OVCAR-3 cells. WB: HepG2, LNCaP, GaMG, A549, MCF7, HeLa, TCMK and NIH/3T3 whole cell lysate. IP: HeLa and HepG2 whole cell lysate.
General notes

This product is sold under License from Bethyl Laboratories, Inc

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA
Concentration information loading...
Purity

Affinity purified
Purification notes

Purified from TCS.
Clonality

Monoclonal
Clone number

BLR086G
Isotype

IgG
Research areas

Western blot - Anti-beta Catenin antibody (ab265591)

All lanes : Anti-beta Catenin antibody [BLR086G] (ab265591) at 1/1000 dilution

Lane 1 : HepG2 whole cell lysate
Lane 2 : LNCaP whole cell lysate
Lane 3 : K562 whole cell lysate
Lane 4 : GaMG whole cell lysate
Lane 5 : Jurkat whole cell lysate
Lane 6 : A549 whole cell lysate
Lane 7 : MCF7 whole cell lysate
Lane 8 : HeLa whole cell lysate
Lane 9 : TCMK whole cell lysate
Lane 10 : NIH/3T3 whole cell lysate

Lysates/proteins at 50 µg per lane.

Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG

Predicted band size: 85 kDa

Exposure time: 10 seconds

Cells prepared using NETN lysis buffer.
Immunocytochemistry - Anti-beta Catenin antibody (ab265591)

Immunocytochemical analysis of formalin-fixed, paraffin-embedded OVCAR-3 (Human ovarian carcinoma cell line) cells labeling beta Catenin with ab265591 at 1/100 dilution. Detection: DAB.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody (ab265591)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human colon carcinoma tissue labeling beta Catenin with ab265591 at 1/100 dilution. Detection: DAB.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin antibody (ab265591)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human plasmacytoma tissue labeling beta Catenin with ab265591 at 1/100 dilution. Detection: DAB.
Immunoprecipitation - Anti-beta Catenin antibody (ab265591)

beta Catenin was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab265591 at 20 µl per reaction. Western blot was performed from the immunoprecipitate using ab265591 at 1/1000 dilution.

Lane 1: ab265591 IP in HeLa whole cell lysate.
Lane 2: Control IgG.

Exposure time: 3 secs.

Cells prepared using NETN lysis buffer.
Immunoprecipitation - Anti-beta Catenin antibody (ab265591)

beta Catenin was immunoprecipitated from 1mg of HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate with ab265591 at 20 µl per reaction. Western blot was performed from the immunoprecipitate using ab265591 at 1/1000 dilution.

Lane 1: ab265591 IP in HeLa whole cell lysate.
Lane 2: Control IgG.

Exposure time: 3 secs.

Cells prepared using NETN lysis buffer.
Anti-beta Catenin antibody [BLR086G] (ab265591)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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