Human N-Cadherin ELISA Kit (ab254512)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 41 pg/ml
- Range: 156.25 pg/ml - 10000 pg/ml
- Sample type: Cell culture extracts, Cell culture supernatant, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Cow, Human
Overview
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Product name
Human N-Cadherin ELISA Kit
See all N Cadherin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 4.4% Inter-assay Sample n Mean SD CV% Serum 3 9.8% -
Sample type
Cell culture supernatant, Serum, Cell culture extracts, Hep Plasma, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
41 pg/ml -
Range
156.25 pg/ml - 10000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 92 81% - 102% Serum 114 113% - 115% Cell culture extracts 97 84% - 108% Hep Plasma 92 89% - 95% EDTA Plasma 108 106% - 109% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Cow, Human -
Product overview
Human N-Cadherin ELISA Kit (ab254512) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of N-Cadherin protein in edta plasma, hep plasma, cell culture extracts, serum, and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human N-Cadherin with 41 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
N-Cadherin is a member of the calcium-dependent adhesion molecule family of classical cadherins encoded by the CDH2 gene. This transmembrane, homophilic glycoprotein often can be found concentrated at adherens junctions in most cell types. The function of N-Cadherin depends much on the cellular contents such as cancer metastasis, interactions with catenins in neutral tissues, and maintaining the structural integrity of the heart by mediating strong adhesion between cardiomyocytes. This kit is raised as an extracellular fragment of N-Cadherin.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human N-Cadherin Capture Antibody 1 x 600µl 10X Human N-Cadherin Detector Antibody 2 vials 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 5BI 1 x 6ml Human N-Cadherin Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density. -
Sequence similarities
Contains 5 cadherin domains. -
Cellular localization
Cell membrane. - Information by UniProt
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Alternative names
- CADH2_HUMAN
- Cadherin 2
- Cadherin 2 N cadherin neuronal
see all -
Database links
- Entrez Gene: 281062 Cow
- Entrez Gene: 1000 Human
- Omim: 114020 Human
- SwissProt: P19022 Human
- Unigene: 464829 Human
- Unigene: 606106 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human N-Cadherin standard curve in Sample Diluent NS.The N-Cadherin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native N-Cadherin in human serum, plasma and cell culture supernatant samples.The concentrations of N-Cadherin were measured in duplicates, interpolated from the N-Cadherin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (EDTA) 50%, plasma (heparin) 50% and HeLa cell culture supernatant 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean N-Cadherin concentration was determined to be 17,850 pg/mL in serum, 19,670 pg/mL in plasma (EDTA) and 12,801 pg/mL in plasma (heparin) and 8391 pg/mL in HeLa cell culture supernatant.
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Interpolated concentrations of native N-Cadherin in human HeLa cell extract based on a 500 µg/mL extract load.The concentrations of N-Cadherin were measured in duplicate and interpolated from the N-Cadherin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean N-Cadherin concentration was determined to be 13,895 pg/mL in HeLa cell extract.
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Sandwich ELISA - Human N-Cadherin ELISA Kit (ab254512)To learn more about the advantages of recombinant antibodies see here.
