Anti-AKR1A1 antibody (ab251727)
Key features and details
- Rabbit polyclonal to AKR1A1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-AKR1A1 antibody
See all AKR1A1 primary antibodies -
Description
Rabbit polyclonal to AKR1A1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig, Chinese hamster, Orangutan
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Immunogen
Recombinant fragment corresponding to Human AKR1A1 aa 100-200.
Database link: P14550 -
Positive control
- IHC-P: Human pancreas tissue. ICC/IF: A431 cells. WB: HEK-293T overexpressing human AKR1A1 with a C-terminal myc-DDK tag, whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AKR1A1 antibody (ab251727)
Paraffin-embedded human pancreas tissue stained for AKR1A1 using ab251727 at 1/200 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-AKR1A1 antibody (ab251727)PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for AKR1A1 (green) using ab251727 at 4 µg/ml in ICC/IF. -
Western blot - Anti-AKR1A1 antibody (ab251727)All lanes : Anti-AKR1A1 antibody (ab251727) at 0.4 µg/ml
Lane 1 : Vector only transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : HEK-293T overexpressing human AKR1A1 with a C-terminal myc-DDK tag, whole cell lysate.
Predicted band size: 37 kDa
