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Anti-NAK/TBK1 antibody [EPR2867(2)-19] (ab109735)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2867(2)-19] to NAK/TBK1
  • Suitable for: WB, IHC-P, IP
  • Knockout validated
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-NAK/TBK1 antibody [EPR2867(2)-19]
    See all NAK/TBK1 primary antibodies

  • Description

    Rabbit monoclonal [EPR2867(2)-19] to NAK/TBK1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa, U937, HepG2, and 293T cell lysates, Human testis tissue, HeLa cells

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-NAK/TBK1 antibody [EPR2867(2)-19] (ab109735)
    Western blot - Anti-NAK/TBK1 antibody [EPR2867(2)-19] (ab109735)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: NAK/TBK1 knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: HepG2 cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab109735 observed at 90 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab109735 was shown to recognize NAK/TBK1 when NAK/TBK1 knockout samples were used, along with additional cross-reactive bands. Wild-type and NAK/TBK1 knockout samples were subjected to SDS-PAGE. ab109735 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAK/TBK1 antibody [EPR2867(2)-19] (ab109735)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAK/TBK1 antibody [EPR2867(2)-19] (ab109735)

    Immunohistochemical analysis of paraffin-embedded human testis tissue using ab109735 at a dilution of 1/250.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-NAK/TBK1 antibody [EPR2867(2)-19] (ab109735)
    Immunoprecipitation - Anti-NAK/TBK1 antibody [EPR2867(2)-19] (ab109735)

    Purified ab109735 at 1/30 dilution (2µg) immunoprecipitating NAK/TBK1 in HeLa whole cell lysate.
    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
    Lane 2 (+): ab109735 + HeLa whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab109735 in HeLa whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/5000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 84 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAK/TBK1 antibody [EPR2867(2)-19] (ab109735)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAK/TBK1 antibody [EPR2867(2)-19] (ab109735)

    ab109735 staining NAK/TBK1 in Human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed and paraffin-embedded, antigen retrieval was by heat mediation in Tris/EDTA buffer pH9. Samples were incubated with primary antibody (1/800). An undiluted HRP-conjugated anti-rabbit IgG was used as the secondary antibody. Tissue counterstained with Hematoxylin.

  • Western blot - Anti-NAK/TBK1 antibody [EPR2867(2)-19] (ab109735)
    Western blot - Anti-NAK/TBK1 antibody [EPR2867(2)-19] (ab109735)
    All lanes : Anti-NAK/TBK1 antibody [EPR2867(2)-19] (ab109735) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : U937 cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 84 kDa

  • Anti-NAK/TBK1 antibody [EPR2867(2)-19] (ab109735)
    Anti-NAK/TBK1 antibody [EPR2867(2)-19] (ab109735)

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