Antibodies, Proteins, Kits and Reagents for Life Science

268 032 ₸ Product size

100 µl 268 032 ₸

Order now and get it on Wednesday March 17, 2021

Key features and details

Mouse monoclonal [GT3612] to LC3B
Suitable for: WB, ICC/IF
Reacts with: Human
Isotype: IgG2a

Product name

Anti-LC3B antibody [GT3612]
See all LC3B primary antibodies
Description

Mouse monoclonal [GT3612] to LC3B
Host species

Mouse
Tested Applications & Species

Application Species
ICC/IF
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide within Human LC3B (N terminal). The exact sequence is proprietary. (Carrier-protein conjugated).
Database link: Q9GZQ8
Positive control
- ICC/IF: HeLa cells treated with 50µM Chloroquine for 24 hr. WB: LC3B transfected HEK-293T whole cell extracts; 3 µM Thapsigargin (12 hr) treated HepG2 whole cell extracts; 50µM Chloroquine (24 hr) treated HeLa whole cell extracts.
General notes

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Application	Species
ICC/IF	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.40
Constituent: 100% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

GT3612
Isotype

IgG2a
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-LC3B antibody [GT3612] (ab243506)

4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells, mock treated (left) and treated with 50 μM Chloroquine for 24 hr (right), stained for LC3B (green) using ab243506 at 1/200 dilution in ICC/IF.

Blue: Hoechst 33342 staining.

Scale bar = 10 μm.
Western blot - Anti-LC3B antibody [GT3612] (ab243506)

All lanes : Anti-LC3B antibody [GT3612] (ab243506) at 1/500 dilution

Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts
Lane 2 : LC3A-transfected HEK-293T whole cell extracts
Lane 3 : LC3B-transfected HEK-293T whole cell extracts

Lysates/proteins at 30 µg per lane.

Predicted band size: 15 kDa

15% SDS-PAGE gel.
Western blot - Anti-LC3B antibody [GT3612] (ab243506)

All lanes : Anti-LC3B antibody [GT3612] (ab243506) at 1/500 dilution

Lane 1 : Untreated HepG2 (human liver hepatocellular carcinoma cell line) whole cell extracts
Lane 2 : 3 µM Thapsigargin (12 hr) treated HepG2 whole cell extracts

Lysates/proteins at 30 µg per lane.

Predicted band size: 15 kDa

15% SDS-PAGE gel.
Western blot - Anti-LC3B antibody [GT3612] (ab243506)

All lanes : Anti-LC3B antibody [GT3612] (ab243506) at 1/500 dilution

Lane 1 : Untreated HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extracts
Lane 2 : 50µM Chloroquine (24 hr) treated HeLa whole cell extracts

Lysates/proteins at 50 µg per lane.

Predicted band size: 15 kDa

15% SDS-PAGE gel.

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