Anti-PDGFR alpha (phospho Y754) antibody (ab5460)
Key features and details
- Rabbit polyclonal to PDGFR alpha (phospho Y754)
- Suitable for: IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-PDGFR alpha (phospho Y754) antibody
See all PDGFR alpha primary antibodies -
Description
Rabbit polyclonal to PDGFR alpha (phospho Y754) -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
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Immunogen
Synthetic peptide corresponding to Human PDGFR alpha.
(Peptide available asab5695) -
Positive control
- IHC: Mouse colo, human brain tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated PDGFR alpha. The final product is generated by affinity chromatography using a PDGFR alpha derived peptide phosphorylated at tyrosine 754. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR alpha (phospho Y754) antibody (ab5460)
Paraffin embedded mouse colon tissue stained for PDGFR alpha (phospho Y754) using ab5460 (right) at 1/20 dilution compared to a negative control section without primary antibody (left), followed by HRP-conjugated secondary antibody in immunohistochemical analysis.
Heat mediated antigen retrieval was performed using 10mM sodium citrate (pH 6.0).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR alpha (phospho Y754) antibody (ab5460)Paraffin embedded human brain tissue stained for PDGFR alpha (phospho Y754) usinf ab5460 (right) at 1/20 dilution compared to a negative control section without primary antibody (left), followed by HRP-conjugated secondary antibody in immunohistochemical analysis.
Heat mediated antigen retrieval was performed using 10mM sodium citrate (pH 6.0).
