Anti-PDGFR alpha (phospho Y849) antibody (ab79318)
Key features and details
- Rabbit polyclonal to PDGFR alpha (phospho Y849)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-PDGFR alpha (phospho Y849) antibody
See all PDGFR alpha primary antibodies -
Description
Rabbit polyclonal to PDGFR alpha (phospho Y849) -
Host species
Rabbit -
Specificity
ab79318 detects endogenous levels of PDGFR alpha only when phosphorylated at tyrosine 849. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
-
Immunogen
Synthetic peptide corresponding to Human PDGFR alpha (phospho Y849).
Sequence:S-N-YP-V-S
-
Positive control
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-PDGFR alpha (phospho Y849) antibody (ab79318)All lanes : Anti-PDGFR alpha (phospho Y849) antibody (ab79318) at 1/500 dilution
Lane 1 : 293 cells extract
Lane 2 : 293 cells extract with Immunizing phosphopeptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 123 kDa
Observed band size: 133 kDa why is the actual band size different from the predicted?
