Recombinant human CDK2 + Cyclin A2 protein (ab196060)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: >= 64% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus, His tag N-Terminus, GST tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant human CDK2 + Cyclin A2 protein
See all CDK2 + Cyclin A2 proteins and peptides -
Biological activity
Specific activity 390 pmol/min/µg.
Kinase buffer containing 1 mM DTT using Histone H1 (0.1 mg/ml) substrate and 20 µM ATP at 30°C for 30 min. The amount of ATP transferred was calculated using Kinase reagents
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Purity
>= 64 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Amino Acid Sequence 1
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Species
Human -
Sequence
ENFQKVEKIGEGTYGVVYKARNKLTGEVVALKKIRLDTETEGVPSTAIRE ISLLKELNH PNIVKLLDVIHTENKLYLVFEFLHQDLKKFMDASALTGI PLPLIKSYLFQLLQGLAFCHS HRVLHRDLKPQNLLINTEGAIKLADFG LARAFGVPVRTYTHEVVTLWYRAPEILLGCKYY STAVDIWSLGCIFAE MVTRRALFPGDSEIDQLFRIFRTLGTPDEVVWPGVTSMPDYKPSF PKW ARQDFSKVVPPLDEDGRSLLSQMLHYDPNKRISAKAALAHPFFQDVTKPV PHLRL -
Predicted molecular weight
61 kDa including tags -
Amino acids
2 to 298 -
Tags
GST tag N-Terminus -
Additional sequence information
(NM_001798)
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Amino Acid Sequence 2
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Species
Human -
Sequence
EVPDYHE DIHTYLREME VKCKPKVGYM KKQPDITNSM RAILVDWLVE VGEEYKLQNE TLHLAVNYID RFLSSMSVLR GKLQLVGTAA MLLASKFEEI YPPEVAEFVY ITDDTYTKKQ VLRMEHLVLK VLTFDLAAPT VNQFLTQYFL HQQPANCKVE SLAMFLGELS LIDADPYLKY LPSVIAGAAF HLALYTVTGQ SWPESLIRKT GYTLESLKPC LMDLHQTYLK APQHAQQSIR EKYKNSKYHG VSLLNPPETL NL -
Predicted molecular weight
34 kDa including tags -
Amino acids
174 to 432 -
Tags
His tag N-Terminus , GST tag N-Terminus -
Additional sequence information
Cyclin A2 (NM_001237)
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Preparation and Storage
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Applications
Functional Studies
SDS-PAGE
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Form
Liquid -
Concentration information loading...
Images
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SDS-PAGE - Recombinant human CDK2 + Cyclin A2 protein (ab196060)
SDS-PAGE analysis of ab196060 on 10% SDS-PAGE gel and stained with Coomassie blue (top band Cyclin A2; bottom band: Cdk2).
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SDS-PAGE - Recombinant human CDK2 + Cyclin A2 protein (ab196060)Specific activity of ab196060
