Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸

Order now and get it on Tuesday March 09, 2021

Anti-Cdk9 antibody [EPR22956-60] (ab261724)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22956-60] to Cdk9
Suitable for: Flow Cyt, WB, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-Cdk9 antibody [EPR22956-60]
See all Cdk9 primary antibodies
Description

Rabbit monoclonal [EPR22956-60] to Cdk9
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Mouse Human
IP	Mouse Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HEK-293T, HeLa, Human heart, Human spleen, C6, RAW264.7, PC-12 and NIH/3T3 lysates. Flow Cyt: HeLa and NIH/3T3 cells. IP: HeLa and NIH/3T3 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.5% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22956-60
Isotype

IgG
Research areas

Western blot - Anti-Cdk9 antibody [EPR22956-60] (ab261724)

All lanes : Anti-Cdk9 antibody [EPR22956-60] (ab261724) at 1/1000 dilution

Lane 1 : C6 (rat glial tumor glial cell), whole cell lysate
Lane 2 : RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate
Lane 3 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
Lanes 1-2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Lane 3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 43 kDa
Observed band size: 42/55 kDa
why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

The 2 isoforms (42KD, 55KD) of CDK9 observed is consistent with what has been described in the literature (PMID: 26766294, 15452830).

Exposure time: 3 minutes
Western blot - Anti-Cdk9 antibody [EPR22956-60] (ab261724)

All lanes : Anti-Cdk9 antibody [EPR22956-60] (ab261724) at 1/1000 dilution

Lane 1 : 293T (human embryonic kidney epithelial cell), whole cell lysate
Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lane 3 : Human heart tissue lysate
Lane 4 : Human spleen tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 43 kDa
Observed band size: 42/55 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST

The 2 isoforms (42KDa, 55KD) of CDK9 observed is consistent with what has been described in the literature (PMID: 26766294, 15452830).

Exposure time: Lanes 1-2: 7.75 seconds Lanes 3-4: 3 minutes
Immunoprecipitation - Anti-Cdk9 antibody [EPR22956-60] (ab261724)

CDK9 was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast) whole cell lysate with ab261724 at 1/30 (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab261724 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 10ug

Lane 2: ab261724 IP in NIH/3T3 whole cell lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab261724 in NIH/3T3 whole cell lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 10 seconds

The 2 isoforms (42KD, 55KD) of CDK9 observed is consistent with what has been described in the literature (PMID: 26766294, 15452830).
Immunoprecipitation - Anti-Cdk9 antibody [EPR22956-60] (ab261724)

CDK9 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with ab261724 at 1/30 (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab261724 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10ug

Lane 2: ab261724 IP in HeLa whole cell lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab261724 in HeLa whole cell lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 10 seconds

The 2 isoforms (42KD, 55KD) of CDK9 observed is consistent with what has been described in the literature (PMID: 26766294, 15452830).
Flow Cytometry - Anti-Cdk9 antibody [EPR22956-60] (ab261724)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling CDK9 with ab261724 at 1/600 (Red) compared with a Rabbit monoclonal IgG (ab172730, Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/200 was used as the secondary antibody.
Flow Cytometry - Anti-Cdk9 antibody [EPR22956-60] (ab261724)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling CDK9 with ab261724 at 1/600 (Red) compared with a Rabbit monoclonal IgG (ab172730, Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/200 was used as the secondary antibody.
Anti-Cdk9 antibody [EPR22956-60] (ab261724)

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