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Anti-Cdk9 antibody [EPR22956-60] - BSA and Azide free (ab261726)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22956-60] to Cdk9 - BSA and Azide free
  • Suitable for: Flow Cyt, WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Cdk9 antibody [EPR22956-60] - BSA and Azide free
    See all Cdk9 primary antibodies

  • Description

    Rabbit monoclonal [EPR22956-60] to Cdk9 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, WB, IPmore details
    Unsuitable for: ChIP,ICC/IF or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: 293T, HeLa, Human heart, Human spleen, C6, RAW264.7, PC-12 and NIH/3T3 lysates. Flow Cyt: HeLa and NIH/3T3 cells. IP: HeLa and NIH/3T3 cells.

  • General notes

    Ab261726 is the carrier-free version of ab261724. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab261726 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-Cdk9 antibody [EPR22956-60] - BSA and Azide free (ab261726)
    Immunoprecipitation - Anti-Cdk9 antibody [EPR22956-60] - BSA and Azide free (ab261726)

    CDK9 was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 10ug with ab261724 at 1/30 (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab261724 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

    Lane 1: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate 10ug

    Lane 2: ab261724 IP in NIH/3T3 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab261724 in NIH/3T3 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds

    The 2 isoforms (42KD, 55KD) of CDK9 observed is consistent with what has been described in the literature (PMID: 26766294, 15452830).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab261724).

  • Immunoprecipitation - Anti-Cdk9 antibody [EPR22956-60] - BSA and Azide free (ab261726)
    Immunoprecipitation - Anti-Cdk9 antibody [EPR22956-60] - BSA and Azide free (ab261726)

    CDK9 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with ab261724 at 1/30 (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab261724 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

    Lane 1: HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10ug

    Lane 2: ab261724 IP in HeLa whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab261724 in HeLa whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 10 seconds

    The 2 isoforms (42KD, 55KD) of CDK9 observed is consistent with what has been described in the literature (PMID: 26766294, 15452830).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab261724).

  • Flow Cytometry - Anti-Cdk9 antibody [EPR22956-60] - BSA and Azide free (ab261726)
    Flow Cytometry - Anti-Cdk9 antibody [EPR22956-60] - BSA and Azide free (ab261726)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling CDK9 with ab261724 at 1/600 (Red) compared with a Rabbit monoclonal IgG (ab172730, Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/200 was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab261724).

  • Flow Cytometry - Anti-Cdk9 antibody [EPR22956-60] - BSA and Azide free (ab261726)
    Flow Cytometry - Anti-Cdk9 antibody [EPR22956-60] - BSA and Azide free (ab261726)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling CDK9 with ab261724 at 1/600 (Red) compared with a Rabbit monoclonal IgG (ab172730, Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/200 was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab261724).

  • Anti-Cdk9 antibody [EPR22956-60] - BSA and Azide free (ab261726)
    Anti-Cdk9 antibody [EPR22956-60] - BSA and Azide free (ab261726)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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