Human CDK4 knockout HeLa cell pellet (ab278889)
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit Human CDK4 knockout HeLa cell pellet 1 vial Human wild-type HeLa cell pellet 1 vial -
Research areas
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Cell type
epithelial -
Disease
Adenocarcinoma -
Gender
Female -
STR Analysis
Amelogenin X D5S818: 11, 12 D13S317: 12, 13.3 D7S820: 8, 12 D16S539: 9, 10 vWA: 16, 18 TH01: 7 TPOX: 8,12 CSF1PO: 9, 10
Images
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Western blot - Human CDK4 knockout HeLa cell pellet (ab278889)Lane 1: Wild-type HeLa cell lysate (20µg)
Lane 2: CDK4 knockout HeLa cell lysate (20µg)
Lanes 1- 2: Merged signal (red and green). Green - ab199728 observed at 34 kDa. Red - loading control ab130007 observed at 124 kDa.
ab199728 Recombinant Anti-Cdk4 antibody [EPR17525] was shown to specifically react with CDK4 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab255378 (knockout cell lysate ab263780) was used. Wild-type and CDK4 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab199728 and Anti-Vinculin antibody [VIN-54] were incubated overnight at 4°C at 1 in 2000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging. -
Western blot - Human CDK4 knockout HeLa cell pellet (ab278889)Lane 1: Wild-type HeLa cell lysate (20µg)
Lane 2: CDK4 knockout HeLa cell lysate (20µg)
Lanes 1- 2: Merged signal (red and green). Green - ab108357 observed at 34 kDa. Red - loading control ab130007 observed at 124 kDa.
ab108357 Recombinant Anti-Cdk4 antibody [EPR4513-32-7] was shown to specifically react with CDK4 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab255378 (knockout cell lysate ab263780) was used. Wild-type and CDK4 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab108357 and Anti-Vinculin antibody [VIN-54] were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging. -
Sanger Sequencing - Human CDK4 knockout HeLa cell pellet (ab278889)Allele-1: 2 bp deletion in exon2; Allele-2: 139 bp insertion in exon2