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Human GSK3A (GSK3 alpha) knockout HEK293T cell pellet (ab278952)

Overview

  • Product name

    Human GSK3A (GSK3 alpha) knockout HEK293T cell pellet
    See all GSK3 alpha kits

  • Product overview

    Abcam’s knockout cell pellets give you access to native proteins, without the need to culture cells. Our knockout cell pellets are prepared from our single-gene knockout cell lines and provide an additional offering to our cell lysates.

    Cells are snap-frozen to provide high quality pellets that are suitable for extraction with alternative lysis buffers or for preparation of lysates from subcellular fractions. Our knockout cell pellets are suitable for a variety of applications, including PCR, gene expression profiling and DNA library preparation.

  • Parental Cell Line

    HEK293T

  • Organism

    Human

  • Mutation description

    Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1 and Insertion of the selection cassette in exon1.

  • Passage number

  • Knockout validation

    Sanger Sequencing, Western Blot (WB)

  • Notes

    Pellet size: 5 million cells/vial.

    This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

  • Tested applications

    Suitable for: WBmore details

Properties

Target

  • Function

    Implicated in the hormonal control of several regulatory proteins including glycogen synthase, MYB and the transcription factor JUN.

  • Sequence similarities

    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.
    Contains 1 protein kinase domain.
  • Information by UniProt

  • Alternative names

    • DKFZp686D0638
    • Glycogen synthase kinase 3 alpha
    • Glycogen synthase kinase-3 alpha
    • GSK 3 alpha
    • GSK 3A
    • GSK-3 alpha
    • Gsk3a
    • GSK3A_HUMAN
    • GSK3alpha
    • Serine/threonine protein kinase GSK3A
    see all

Properties

Images

  • Western blot - Human GSK3A (GSK3 alpha) knockout HEK293T cell pellet (ab278952)
    Western blot - Human GSK3A (GSK3 alpha) knockout HEK293T cell pellet (ab278952)

    Lane 1:Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (20 ug)
    Lane 2:GSK3A knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (20 ug)
    Lane 3:HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (20 ug)
    Lane 4:HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate (20 ug)

    ab40870 was shown to specifically react with GSK3 alpha in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266742 (knockout cell lysate ab257979) was used. Wild-type and GSK3 alpha knockout samples were subjected to SDS-PAGE. ab40870 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4oC at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Human GSK3A (GSK3 alpha) knockout HEK293T cell pellet (ab278952)
    Western blot - Human GSK3A (GSK3 alpha) knockout HEK293T cell pellet (ab278952)

    Lane 1:Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (20 ug)
    Lane 2:GSK3A knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (20 ug)
    Lane 3:HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (20 ug)
    Lane 4:HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate (20 ug)

    ab78664 was shown to specifically react with GSK3 alpha in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266742 (knockout cell lysate ab257979) was used. Wild-type and GSK3 alpha knockout samples were subjected to SDS-PAGE. ab78664 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4oC at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Sanger Sequencing - Human GSK3A (GSK3 alpha) knockout HEK293T cell pellet (ab278952)
    Sanger Sequencing - Human GSK3A (GSK3 alpha) knockout HEK293T cell pellet (ab278952)

    Allele-1: 1 bp deletion in exon1

     

  • Sanger Sequencing - Human GSK3A (GSK3 alpha) knockout HEK293T cell pellet (ab278952)
    Sanger Sequencing - Human GSK3A (GSK3 alpha) knockout HEK293T cell pellet (ab278952)

    Allele-2: Insertion of the selection cassette in exon1

     

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