Anti-Cdk2 antibody (ab232753)
Key features and details
- Rabbit polyclonal to Cdk2
- Suitable for: WB, IHC-P
- Reacts with: Human, Pig
- Isotype: IgG
Overview
-
Product name
Anti-Cdk2 antibody
See all CDK2 primary antibodies -
Description
Rabbit polyclonal to Cdk2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human, Pig
Predicted to work with: Mouse, Rat, Cow, Chinese hamster
-
Immunogen
Recombinant fragment (His-tag) corresponding to Human CDK2 aa 50-200. Expressed in E.coli. N-terminal tag.
Database link: P24941 -
Positive control
- IHC-P: Human liver, liver cancer, breast cancer and colorectal cancer tissues. WB: HeLa, HepG2 and K562 cell lysates; Pig liver lysate; Recombinant human Cdk2 protein.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab232753 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-Cdk2 antibody (ab232753)All lanes : Anti-Cdk2 antibody (ab232753) at 2 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate
Lane 3 : K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate
Lane 4 : Pig liver lysate
Predicted band size: 34 kDa
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk2 antibody (ab232753)Paraffin-embedded human liver tissue stained for Cdk2 using ab232753 at 20 µg/ml in immunohistochemical analysis. DAB staining.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk2 antibody (ab232753)Paraffin-embedded human liver cancer tissue stained for Cdk2 using ab232753 at 20 µg/ml in immunohistochemical analysis. DAB staining.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk2 antibody (ab232753)Paraffin-embedded human breast cancer tissue stained for Cdk2 using ab232753 at 20 µg/ml in immunohistochemical analysis, followed by HRP-Linked Goat Anti-Rabbit IgG Polyclonal Antibody at 2 µg/ml. DAB staining.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cdk2 antibody (ab232753)Paraffin-embedded human colorectal cancer tissue stained for Cdk2 using ab232753 at 20 µg/ml in immunohistochemical analysis, followed by HRP-Linked Goat Anti-Rabbit IgG Polyclonal Antibody at 2 µg/ml. DAB staining.
-
Western blot - Anti-Cdk2 antibody (ab232753)Anti-Cdk2 antibody (ab232753) at 2 µg/ml + Recombinant human Cdk2 protein
Predicted band size: 34 kDa
