Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Hexokinase 1 antibody [EPR10135(B)] - BSA and Azide free (ab249143)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10135(B)] to Hexokinase 1 - BSA and Azide free
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Hexokinase 1 antibody [EPR10135(B)] - BSA and Azide free
    See all Hexokinase 1 primary antibodies

  • Description

    Rabbit monoclonal [EPR10135(B)] to Hexokinase 1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: MCF7 and HEK-293T cell lysates.

  • General notes

    ab249143 is the carrier-free version of ab154839. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab249143 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Hexokinase 1 antibody [EPR10135(B)] - BSA and Azide free (ab249143)
    Western blot - Anti-Hexokinase 1 antibody [EPR10135(B)] - BSA and Azide free (ab249143)
    All lanes : Anti-Hexokinase 1 antibody [EPR10135(B)] (ab154839) at 1/1000 dilution

    Lane 1 : Wild-type HEK-293T cell lysate
    Lane 2 : HK1 knockout HeLa cell lysate
    Lane 3 : MCF7 cell lysate
    Lane 4 : HEPG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 102 kDa
    Observed band size: 102 kDa



    This data was developed using the same antibody clone in a different buffer formulation (ab154839).

    Lanes 1-4: Merged signal (red and green). Green - ab154839 observed at 102 kDa. Red - loading control, ab8245 observed at 37 kDa.

    ab154839 Anti-Hexokinase 1 antibody [EPR10135(B)] was shown to specifically react with Hexokinase 1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab267279 (knockout cell lysate ab257161) was used. Wild-type and Hexokinase 1 knockout samples were subjected to SDS-PAGE. ab154839 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

     

     

  • Anti-Hexokinase 1 antibody [EPR10135(B)] - BSA and Azide free (ab249143)
    Anti-Hexokinase 1 antibody [EPR10135(B)] - BSA and Azide free (ab249143)

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