Anti-CD9 antibody [MZ3] - BSA and Azide free (ab239597)
![Anti-CD9 antibody [MZ3] - BSA and Azide free (ab239597)](https://www.abcam.com/ps/products/239/ab239597/Images/ab239597-1-antiCD9ab235119-flow-cytometryC57BL6mousebonemarrow.jpg "Anti-CD9 antibody [MZ3] - BSA and Azide free (ab239597)")
Key features and details
- Rat monoclonal [MZ3] to CD9 - BSA and Azide free
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Isotype: IgG2a
Overview
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Product name
Anti-CD9 antibody [MZ3] - BSA and Azide free
See all CD9 primary antibodies -
Description
Rat monoclonal [MZ3] to CD9 - BSA and Azide free -
Host species
Rat -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Mouse
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Immunogen
Tissue, cells or virus corresponding to CD9. Mouse B cells.
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Positive control
- Flow Cyt: C57BL/6 bone marrow cells.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
MZ3 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Images
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Flow Cytometry - Anti-CD9 antibody [MZ3] - BSA and Azide free (ab239597)
This data was developed using the same antibody clone in a different buffer formulation (ab235119)
C57BL/6 mouse bone marrow cells stained with ab235119 (right) or Rat IgG2aκ (ab18450) isotype (left). C57BL/6 mouse bone marrow cells were incubated for 30 min on ice in 10 % mouse serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab235119) or Rat IgG2aκ (ab18450) (1x106 in 100µl at 0.04 µg/ml) for 30 min on ice.
The secondary antibody Goat anti-rat IgG H&L (Alexa Fluor ® 488, pre-adsorbed) (ab150165) was used at 1/2000 dilution for 30 min at 4°C. The cells were simultaneously stained with CD11b antibody.
Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on viable single cells.
