Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-FGF2 antibody [EPR20145-219] - Low endotoxin, Azide free (ab222932)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20145-219] to FGF2 - Low endotoxin, Azide free
  • Suitable for: ELISA, Flow Cyt (Intra), ICC/IF, IP, WB
  • Reacts with: Human, Recombinant fragment

Overview

  • Product name

    Anti-FGF2 antibody [EPR20145-219] - Low endotoxin, Azide free
    See all FGF2 primary antibodies

  • Description

    Rabbit monoclonal [EPR20145-219] to FGF2 - Low endotoxin, Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ELISA, Flow Cyt (Intra), ICC/IF, IP, WBmore details

  • Species reactivity

    Reacts with: Human, Recombinant fragment

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Recombinant human FGF2 active protein (aa143-288); K562, U-87 MG and SK-OV-3 whole cell lysates; Human fetal kidney, prostate, fetal heart and testis lysates. ICC/IF: U-87 MG and SK-OV-3 cells. Flow Cyt (intra): K562 cells. IP: K562 whole cell lysate.

  • General notes

    ab222932 is the carrier-free version of ab208687.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

Properties

Images

  • Immunoprecipitation - Anti-FGF2 antibody [EPR20145-219] - Low endotoxin, Azide free (ab222932)
    Immunoprecipitation - Anti-FGF2 antibody [EPR20145-219] - Low endotoxin, Azide free (ab222932)

    FGF2 was immunoprecipitated from 0.35 mg of K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate with ab208687 at 1/30 dilution.

    Western blot was performed from the immunoprecipitate using ab208687 at 1/500 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: K562 whole cell lysate 10µg (Input).

    Lane 2: ab208687 IP in K562 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab208687 in K562 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208687).

  • Immunocytochemistry/ Immunofluorescence - Anti-FGF2 antibody [EPR20145-219] - Low endotoxin, Azide free (ab222932)
    Immunocytochemistry/ Immunofluorescence - Anti-FGF2 antibody [EPR20145-219] - Low endotoxin, Azide free (ab222932)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cells labeling FGF2 with ab208687 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing nuclear and weak cytoplasmic staining on U-87 MG cell line.

    The nuclear counterstain is DAPI (blue).

    Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208687).

  • Immunocytochemistry/ Immunofluorescence - Anti-FGF2 antibody [EPR20145-219] - Low endotoxin, Azide free (ab222932)
    Immunocytochemistry/ Immunofluorescence - Anti-FGF2 antibody [EPR20145-219] - Low endotoxin, Azide free (ab222932)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SK-OV-3 (Human ovarian cancer cell line) cells labeling FGF2 with ab208687 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing nuclear weak cytoplasmic staining on SK-OV-3 cell line.

    The nuclear counterstain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208687).

  • Flow Cytometry - Anti-FGF2 antibody [EPR20145-219] - Low endotoxin, Azide free (ab222932)
    Flow Cytometry - Anti-FGF2 antibody [EPR20145-219] - Low endotoxin, Azide free (ab222932)

    Flow cytometric analysis of 4% paraformaldehyde-fixed K562 (Human chronic myelogenous leukemia cell line from bone marrow) cells labeling FGF2 with ab208687 at 1/600 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208687).

  • ELISA - Anti-FGF2 antibody [EPR20145-219] - Low endotoxin, Azide free (ab222932)
    ELISA - Anti-FGF2 antibody [EPR20145-219] - Low endotoxin, Azide free (ab222932)

    This data was developed using ab208687, the same antibody clone in a different buffer formulation.

    ELISA analysis of Mouse FGF2 recombinant protein at 1000 ng/ml with ab208687. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
  • Anti-FGF2 antibody [EPR20145-219] - Low endotoxin, Azide free (ab222932)
    Anti-FGF2 antibody [EPR20145-219] - Low endotoxin, Azide free (ab222932)

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