Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-FGF2 antibody [EPR20145-219] (ab208687)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20145-219] to FGF2
  • Suitable for: Flow Cyt, WB, IP, ICC/IF, ELISA
  • Reacts with: Human, Recombinant fragment

Overview

  • Product name

    Anti-FGF2 antibody [EPR20145-219]
    See all FGF2 primary antibodies

  • Description

    Rabbit monoclonal [EPR20145-219] to FGF2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ELISA
    Recombinant fragment
    Flow Cyt
    Human
    ICC/IF
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Recombinant human FGF2 active protein (aa143-288); K562, U-87 MG and SK-OV-3 whole cell lysates; Human fetal kidney, prostate, fetal heart and testis lysates. ICC/IF: U-87 MG and SK-OV-3 cells. Flow Cyt: K562 cells. IP: K562 whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-FGF2 antibody [EPR20145-219] (ab208687)
    Western blot - Anti-FGF2 antibody [EPR20145-219] (ab208687)
    All lanes : Anti-FGF2 antibody [EPR20145-219] (ab208687) at 1/1000 dilution

    Lane 1 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
    Lane 2 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
    Lane 3 : SK-OV-3 (Human ovarian cancer cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 30 kDa
    Observed band size: 18,22,24 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 3 minutes; Lane 2: 15 seconds; Lane 3: 5 seconds.

    The MW observed is consistent with the literatures (PMID:18850066, PMID: 24827991).

     

     

  • Immunocytochemistry/ Immunofluorescence - Anti-FGF2 antibody [EPR20145-219] (ab208687)
    Immunocytochemistry/ Immunofluorescence - Anti-FGF2 antibody [EPR20145-219] (ab208687)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cells labeling FGF2 with ab208687 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing nuclear and weak cytoplasmic staining on U-87 MG cell line.

    The nuclear counterstain is DAPI (blue).

    Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

  • Western blot - Anti-FGF2 antibody [EPR20145-219] (ab208687)
    Western blot - Anti-FGF2 antibody [EPR20145-219] (ab208687)
    Anti-FGF2 antibody [EPR20145-219] (ab208687) at 1/10000 dilution + Recombinant human FGF2 active protein (aa143-288) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 30 kDa
    Observed band size: 15 kDa why is the actual band size different from the predicted?


    Exposure time: 1 second


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-FGF2 antibody [EPR20145-219] (ab208687)
    Western blot - Anti-FGF2 antibody [EPR20145-219] (ab208687)
    All lanes : Anti-FGF2 antibody [EPR20145-219] (ab208687) at 1/1000 dilution

    Lane 1 : Human fetal kidney lysate
    Lane 2 : Human prostate lysate
    Lane 3 : Human fetal heart lysate
    Lane 4 : Human testis lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/100000 dilution

    Predicted band size: 30 kDa
    Observed band size: 18,22,24 kDa why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The MW observed is consistent with the literatures (PMID:18850066, PMID: 24827991).

  • Immunocytochemistry/ Immunofluorescence - Anti-FGF2 antibody [EPR20145-219] (ab208687)
    Immunocytochemistry/ Immunofluorescence - Anti-FGF2 antibody [EPR20145-219] (ab208687)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SK-OV-3 (Human ovarian cancer cell line) cells labeling FGF2 with ab208687 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

    Confocal image showing nuclear weak cytoplasmic staining on SK-OV-3 cell line.

    The nuclear counterstain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

  • Flow Cytometry - Anti-FGF2 antibody [EPR20145-219] (ab208687)
    Flow Cytometry - Anti-FGF2 antibody [EPR20145-219] (ab208687)

    Flow cytometric analysis of 4% paraformaldehyde-fixed K562 (Human chronic myelogenous leukemia cell line from bone marrow) cells labeling FGF2 with ab208687 at 1/600 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

  • Immunoprecipitation - Anti-FGF2 antibody [EPR20145-219] (ab208687)
    Immunoprecipitation - Anti-FGF2 antibody [EPR20145-219] (ab208687)

    FGF2 was immunoprecipitated from 0.35 mg of K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate with ab208687 at 1/30 dilution.

    Western blot was performed from the immunoprecipitate using ab208687 at 1/500 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: K562 whole cell lysate 10µg (Input).

    Lane 2: ab208687 IP in K562 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab208687 in K562 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

  • ELISA - Anti-FGF2 antibody [EPR20145-219] (ab208687)
    ELISA - Anti-FGF2 antibody [EPR20145-219] (ab208687)

    ELISA analysis of Mouse FGF2 recombinant protein at 1000 ng/ml with ab208687. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.

  • Anti-FGF2 antibody [EPR20145-219] (ab208687)
    Anti-FGF2 antibody [EPR20145-219] (ab208687)

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