Anti-FGF2 antibody [EPR20145-219] - BSA and Azide free (ab271983)
![Anti-FGF2 antibody [EPR20145-219] - BSA and Azide free (ab271983)](https://www.abcam.com/ps/products/271/ab271983/Images/ab271983-4-ab208687267921antifgf2antibodyepr20145219immunofluorescence.jpg "Anti-FGF2 antibody [EPR20145-219] - BSA and Azide free (ab271983)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20145-219] to FGF2 - BSA and Azide free
- Suitable for: Indirect ELISA, Flow Cyt (Intra), IP, ICC/IF, WB
- Reacts with: Human, Recombinant fragment
Overview
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Product name
Anti-FGF2 antibody [EPR20145-219] - BSA and Azide free
See all FGF2 primary antibodies -
Description
Rabbit monoclonal [EPR20145-219] to FGF2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Indirect ELISA, Flow Cyt (Intra), IP, ICC/IF, WBmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Recombinant human FGF2 active protein (aa143-288); K562, U-87 MG and SK-OV-3 whole cell lysates; Human fetal kidney, prostate, fetal heart and testis lysates. ICC/IF: U-87 MG and SK-OV-3 cells. Flow Cyt (intra): K562 cells. IP: K562 whole cell lysate.
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General notes
ab271983 is the carrier-free version of ab208687.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20145-219 -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-FGF2 antibody [EPR20145-219] (ab271983)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cells labeling FGF2 with ab208687 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing nuclear and weak cytoplasmic staining on U-87 MG cell line.
The nuclear counterstain is DAPI (blue).
Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208687). -
![Immunocytochemistry/ Immunofluorescence - Anti-FGF2 antibody [EPR20145-219] (ab271983)](https://www.abcam.com/ps/products/271/ab271983/Images/ab271983-3-ab208687267920antifgf2antibodyepr20145219immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-FGF2 antibody [EPR20145-219] (ab271983)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized SK-OV-3 (Human ovarian cancer cell line) cells labeling FGF2 with ab208687 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing nuclear weak cytoplasmic staining on SK-OV-3 cell line.
The nuclear counterstain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208687). -
![Flow Cytometry - Anti-FGF2 antibody [EPR20145-219] (ab271983)](https://www.abcam.com/ps/products/271/ab271983/Images/ab271983-2-ab208687267919antifgf2antibodyepr20145219flowcytometry.jpg)
Flow Cytometry - Anti-FGF2 antibody [EPR20145-219] (ab271983)Flow cytometric analysis of 4% paraformaldehyde-fixed K562 (Human chronic myelogenous leukemia cell line from bone marrow) cells labeling FGF2 with ab208687 at 1/600 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208687). -
![Immunoprecipitation - Anti-FGF2 antibody [EPR20145-219] (ab271983)](https://www.abcam.com/ps/products/271/ab271983/Images/ab271983-1-ab208687267918antifgf2antibodyepr20145219immunoprecipitation.jpg)
Immunoprecipitation - Anti-FGF2 antibody [EPR20145-219] (ab271983)FGF2 was immunoprecipitated from 0.35 mg of K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate with ab208687 at 1/30 dilution.
Western blot was performed from the immunoprecipitate using ab208687 at 1/500 dilution.
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.
Lane 1: K562 whole cell lysate 10µg (Input).
Lane 2: ab208687 IP in K562 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab208687 in K562 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208687). -
![ELISA - Anti-FGF2 antibody [EPR20145-219] - BSA and Azide free (ab271983)](https://www.abcam.com/ps/products/271/ab271983/Images/ab271983-451053-anti-fgf2-antibody-epr20145-219-elisa-mouse-fgf2.png)
ELISA - Anti-FGF2 antibody [EPR20145-219] - BSA and Azide free (ab271983)This data was developed using ab208687, the same antibody clone in a different buffer formulation.
ELISA analysis of Mouse FGF2 recombinant protein at 1000 ng/ml with ab208687. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
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![Anti-FGF2 antibody [EPR20145-219] - BSA and Azide free (ab271983)](https://www.abcam.com/ps/products/271/ab271983/Images/ab271983-5-benefits-of-recombinant-antibodies.png)
Anti-FGF2 antibody [EPR20145-219] - BSA and Azide free (ab271983)
