Human FGF basic ELISA Kit (FGF2) (ab99980)
Key features and details
- Sensitivity: 50 pg/ml
- Range: 102.4 pg/ml - 10000 pg/ml
- Sample type: Cell culture extracts, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 120X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 2X Cell Lysis Buffer 1 x 5ml 5X Assay Diluent 1 x 15ml 5X Sample Diluent Buffer 1 x 10ml Biotinylated anti-Human FGF basic 2 vials FGF basic Microplate (12 x 8 wells) 1 unit Recombinant Human FGF basic Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
-
Relevance
FGF basic (FGF2) is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. FGF2 is involved in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. FGF2 mRNA contains multiple polyadenylation sites, and is alternatively translated from AUG and non-AUG (CUG) initiation codons resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. -
Alternative names
- Basic fibroblast growth factor
- BFGF
- FGF 2
see all -
Database links
- Entrez Gene: 2247 Human
- Omim: 134920 Human
- SwissProt: P09038 Human
- Unigene: 284244 Human
Images
-
FGF basic (FGF2) Human ELISA Kit (ab99980)
Standard curve: mean of duplicates (+/- SD) with background reads subtracted
-
FGF basic (FGF2) Human ELISA Kit (ab99980)FGF2 measured in tissue lysates showing quantity (pg) per 1 mg of extracted protein. Protein concentration for samples varied from 7 mg/mL to 17 mg/mL. Samples were diluted 200-2000 fold.
-
FGF basic (FGF2) Human ELISA Kit (ab99980)FGF2 measured in cell culture lysates showing quantity (pg) per 1 mln cells. Samples with the concentration of 1e7 cells/mL were used. Samples were diluted 200-2000 fold.
