Human FGF basic ELISA Kit (FGF2) (ab99979)
Key features and details
- Sensitivity: 50 pg/ml
- Range: 102.4 pg/ml - 10000 pg/ml
- Sample type: Cell culture supernatant, Cell Lysate, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 120X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Human FGF basic 2 vials FGF basic Microplate (12 x 8 wells) 1 unit Recombinant Human FGF basic Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Relevance
FGF basic (FGF2) is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. FGF2 is involved in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. FGF2 mRNA contains multiple polyadenylation sites, and is alternatively translated from AUG and non-AUG (CUG) initiation codons resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. -
Alternative names
- Basic fibroblast growth factor
- BFGF
- FGF 2
see all -
Database links
- Entrez Gene: 2247 Human
- Omim: 134920 Human
- SwissProt: P09038 Human
- Unigene: 284244 Human
Images
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FGF2 measured in cell lysates showing quantity (pg) per mg protein present in tested cells
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FGFbasic measured in biological fluids showing quantity (pg) per mL of tested sample -
Typical Standard CurveRepresentative Standard Curve using ab99979.
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Typical Standard CurveRepresentative Standard Curve using ab99979.
