Antibodies, Proteins, Kits and Reagents for Life Science

328 339 ₸ Product size

100 µl 328 339 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP1735] to FGF2
Suitable for: WB, IP, IHC-P, Sandwich ELISA
Reacts with: Human

Product name

Anti-FGF2 antibody [EP1735]
See all FGF2 primary antibodies
Description

Rabbit monoclonal [EP1735] to FGF2
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-P	Human
IP	Human
sELISA	Recombinant fragment
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human FGF2 aa 100-200. The exact sequence is proprietary.
Positive control
- WB: K-562, and HeLa lysates. IHC-P: human breast, prostate and cerebrum tissue. IP: HeLa cells.
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP1735
Isotype

IgG
Research areas

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF2 antibody [EP1735] (ab92337)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast tissue sections labeling FGF2 with Purified ab92337 at 1:2400 dilution (0.51 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunoprecipitation - Anti-FGF2 antibody [EP1735] (ab92337)

ab92337 (Purified) at 1:70 dilution (2 µg) immunoprecipitating FGF2 in HeLa whole cell lysate.
Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10 µg
Lane 2 (+): ab92337 & HeLa whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab92337 in HeLa whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.
Western blot - Anti-FGF2 antibody [EP1735] (ab92337)

Anti-FGF2 antibody [EP1735] (ab92337) at 1/5000 dilution (Purified) + HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 31 kDa
Observed band size: 17-30 kDa
why is the actual band size different from the predicted?

ab92337 recoginzes 3 isoforms of FGF2
Western blot - Anti-FGF2 antibody [EP1735] (ab92337)

Anti-FGF2 antibody [EP1735] (ab92337) at 1/1000 dilution (Purified) + K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates at 15 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 31 kDa
Observed band size: 17-30 kDa why is the actual band size different from the predicted?

ab92337 recoginzes 3 isoforms of FGF2
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF2 antibody [EP1735] (ab92337)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate tissue sections labeling FGF2 with purified ab92337 at 1/1000 dilution (1.3 µg/ml). Heat mediated antigen retrieval was performed. ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody.

Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF2 antibody [EP1735] (ab92337)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebrum tissue sections labeling FGF2 with purified ab92337 at 1/1000 dilution (1.3 µg/ml). Heat mediated antigen retrieval was performed. ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody.

Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Sandwich ELISA - Anti-FGF2 antibody [EP1735] (ab92337)

Standard Curve for FGF basic (Analyte: FGF basic protein (Active) (ab9596)); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [AS24] to FGF basic (ab17279) at 1µg/ml and Detector Antibody Rabbit monoclonal [EP1735] to FGF basic (ab92337) at 0.5µg/ml.
Anti-FGF2 antibody [EP1735] (ab92337)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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