Anti-C5a antibody [7L11] (ab194637)
Key features and details
- Rat monoclonal [7L11] to C5a
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG2a
Overview
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Product name
Anti-C5a antibody [7L11]
See all C5a primary antibodies -
Description
Rat monoclonal [7L11] to C5a -
Host species
Rat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment corresponding to Mouse C5a aa 650-800.
Database link: P06684 -
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Lyophilized:Add 200 µl sterile PBS. Reconstituted antibody can be aliquoted and stored frozen atStorage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.Storage buffer
Constituent: 100% PBSConcentration information loading...Purity
Protein A/G purifiedClonality
MonoclonalClone number
7L11Isotype
IgG2aResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab194637 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use at an assay dependent concentration. Predicted molecular weight: 115 kDa. Target
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Relevance
Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process. Binding to the receptor C5AR1 induces a variety of responses including intracellular calcium release, contraction of smooth muscle, increased vascular permeability, and histamine release from mast cells and basophilic leukocytes. C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation.
Datasheets and documents
References (3)
ab194637 has been referenced in 3 publications.
- Li X et al. Deficiency of Mouse FHR-1 Homolog, FHR-E, Accelerates Sepsis, and Acute Kidney Injury Through Enhancing the LPS-Induced Alternative Complement Pathway. Front Immunol 11:1123 (2020). PubMed: 32636836
- Wang B et al. Complement depletion with cobra venom factor alleviates acute hepatic injury induced by ischemia-reperfusion. Mol Med Rep 18:4523-4529 (2018). PubMed: 30221740
- Feng F et al. PVT1 regulates inflammation and cardiac function via the MAPK/NF-?B pathway in a sepsis model. Exp Ther Med 16:4471-4478 (2018). PubMed: 30546393