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Immunology Innate Immunity Macrophage / Inflamm.

Anti-C5a antibody [7L11] (ab194637)

Price and availability

274 732 ₸

Availability

Order now and get it on Thursday February 25, 2021

Anti-C5a antibody [7L11] (ab194637)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rat monoclonal [7L11] to C5a
  • Suitable for: WB
  • Reacts with: Mouse
  • Isotype: IgG2a

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Overview

  • Product name

    Anti-C5a antibody [7L11]
    See all C5a primary antibodies
  • Description

    Rat monoclonal [7L11] to C5a
  • Host species

    Rat
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Recombinant fragment corresponding to Mouse C5a aa 650-800.
    Database link: P06684

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Lyophilized:Add 200 µl sterile PBS. Reconstituted antibody can be aliquoted and stored frozen at
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: 100% PBS
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Clonality

    Monoclonal
  • Clone number

    7L11
  • Isotype

    IgG2a
  • Research areas

    • Immunology
    • Innate Immunity
    • Macrophage / Inflamm.
    • Immunology
    • Innate Immunity
    • Complement
    • MAC
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR
    • Cell Biology
    • Other Antibodies
    • Oxidative Stress
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Oxidative stress

Associated products

  • Compatible Secondaries

    • Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)
    • Goat Anti-Rat IgG H&L (HRP) (ab205720)
  • Isotype control

    • Rat IgG2a, kappa monoclonal [RTK2758] - Isotype Control (ab18450)
  • Recombinant Protein

    • Recombinant Mouse C5 protein (ab151847)

Applications

Our Abpromise guarantee covers the use of ab194637 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 115 kDa.

Target

  • Relevance

    Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process. Binding to the receptor C5AR1 induces a variety of responses including intracellular calcium release, contraction of smooth muscle, increased vascular permeability, and histamine release from mast cells and basophilic leukocytes. C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation.

Images

  • Western blot - Anti-C5a antibody [7L11] (ab194637)
    Western blot - Anti-C5a antibody [7L11] (ab194637)
    Anti-C5a antibody [7L11] (ab194637) + Mouse serum C5a

    Predicted band size: 115 kDa

Protocols

  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (3)

    Publishing research using ab194637? Please let us know so that we can cite the reference in this datasheet.

    ab194637 has been referenced in 3 publications.

    • Li X  et al. Deficiency of Mouse FHR-1 Homolog, FHR-E, Accelerates Sepsis, and Acute Kidney Injury Through Enhancing the LPS-Induced Alternative Complement Pathway. Front Immunol 11:1123 (2020). PubMed: 32636836
    • Wang B  et al. Complement depletion with cobra venom factor alleviates acute hepatic injury induced by ischemia-reperfusion. Mol Med Rep 18:4523-4529 (2018). PubMed: 30221740
    • Feng F  et al. PVT1 regulates inflammation and cardiac function via the MAPK/NF-?B pathway in a sepsis model. Exp Ther Med 16:4471-4478 (2018). PubMed: 30546393

    Images

    • Western blot - Anti-C5a antibody [7L11] (ab194637)
      Western blot - Anti-C5a antibody [7L11] (ab194637)
      Anti-C5a antibody [7L11] (ab194637) + Mouse serum C5a

      Predicted band size: 115 kDa

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Alternative products to Anti-C5a antibody [7L11] (ab194637)

    •  
    • HRP Anti-C5 antibody (ab193124)

      Applications:

    •  
    • Product image

      Anti-C5 antibody (ab193295)

      Applications: IHC-P

    •  
    • Anti-C5 / C5a antibody [MM0608-6H13] (ab135187)

      Applications:

    •  
    • Biotin Anti-C5 antibody (ab193113)

      Applications:

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