Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-FGF13 antibody [N235/22] (ab186300)

Key features and details

  • Mouse monoclonal [N235/22] to FGF13
  • Suitable for: WB, ICC/IF
  • Reacts with: Rat, Human
  • Isotype: IgG2b

Overview

  • Product name

    Anti-FGF13 antibody [N235/22]

  • Description

    Mouse monoclonal [N235/22] to FGF13

  • Host species

    Mouse

  • Specificity

    ab186300 will not recognize isoforms 2, 3, 4 or 5 of human FGF13. It cross-reacts with FGF12 and FGF14. It has 100% identity with human, mouse and rat FGF12; 94% identity with human, mouse and rat FGF14; 81% identity to human and mouse FGF11.

  • Tested applications

    Suitable for: WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Xenopus laevis, Orangutan

  • Immunogen

    Synthetic peptide corresponding to Human FGF13 aa 1-100 (N terminal).
    Database link: Q92913

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: Rat brain lysate. ICC/IF: SK-N-BE cells.

  • General notes

    1μg/mL of ab186300 was sufficient for detection of FGF13 in 20 μg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

    The clone number has been updated from S235-22 to N235/22, both clone numbers name the same antibody clone.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.1% Sodium azide
    Constituents: 50% Glycerol (glycerin, glycerine), PBS
  • Concentration information loading...

  • Purity

    Protein G purified

  • Clonality

    Monoclonal

  • Clone number

    N235/22

  • Isotype

    IgG2b

  • Research areas

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-FGF13 antibody [N235/22] (ab186300)
    Immunocytochemistry/ Immunofluorescence - Anti-FGF13 antibody [N235/22] (ab186300)

    SK-N-BE (Human neuroblastoma cell line) cells stained for FGF13 using ab186300 at a dilution of 1/100 (1 hour at RT) in ICC/IF.

    Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 1 hour at RT.

    Cells were fixed with 4% formaldehyde for 15 minutes at RT.

  • Western blot - Anti-FGF13 antibody [N235/22] (ab186300)
    Western blot - Anti-FGF13 antibody [N235/22] (ab186300)
    Anti-FGF13 antibody [N235/22] (ab186300) at 1/200 dilution + Rat brain membrane lysate at 15 µg

    Secondary
    Goat Anti-Mouse IgG: HRP at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 28 kDa


    Exposure time: 6 minutes


     Block: 2% BSA and 2% skimmed milk in 1X TBST.

    Incubated with primary antibody for 16 hours at 4°C.

    Incubated with secondary antibody for 1 hour at RT.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-FGF13 antibody [N235/22] (ab186300)

© 2021 Astana Biomed Group LLP. All rights reserved.