Anti-ROCK2 antibody - C-terminal (ab228000)
Key features and details
- Rabbit polyclonal to ROCK2 - C-terminal
- Suitable for: WB, IP, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-ROCK2 antibody - C-terminal
See all ROCK2 primary antibodies -
Description
Rabbit polyclonal to ROCK2 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow, Rhesus monkey
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Immunogen
Synthetic peptide within Human ROCK2 (C terminal). The exact sequence is proprietary. Conjugated to a protein carrier.
Database link: O75116 -
Positive control
- WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts. IHC-P: Mouse brain tissue. IP: HeLa whole cell extracts. ICC/IF: A431 cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ROCK2 antibody - C-terminal (ab228000)All lanes : Anti-ROCK2 antibody - C-terminal (ab228000) at 1/4000 dilution
Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lanes 2-3 : ROCK2 shRNA transfected HEK-293T whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 161 kDa5% SDS-PAGE gel.
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Immunocytochemistry/ Immunofluorescence - Anti-ROCK2 antibody - C-terminal (ab228000)A431 (human epidermoid carcinoma cell line) cells stained for ROCK2 (green) using ab228000 at 1/500 dilution in ICC/IF. Cells were fixed in ice-cold methanol for 5 minutes.
Blue: Hoechst 33342 staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ROCK2 antibody - C-terminal (ab228000)Paraffin-embedded mouse brain tissue stained for ROCK2 using ab228000 at 1/500 dilution in immunohistochemical analysis.
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Immunoprecipitation - Anti-ROCK2 antibody - C-terminal (ab228000)ROCK2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract with 5 µg ab228000. Western blot was performed from the immunoprecipitate using ab228000. Anti-Rabbit IgG was used as a secondary reagent.
Lane 1: HeLa whole cell extract.
Lane 2: Control IgG IP in HeLa whole cell extract.
Lane 3: ab228000 IP in HeLa whole cell extract.
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Western blot - Anti-ROCK2 antibody - C-terminal (ab228000)All lanes : Anti-ROCK2 antibody - C-terminal (ab228000) at 1/5000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 161 kDa5% SDS-PAGE gel.
