Human RIPK2 (RIP2) knockout HeLa cell lysate (ab258636)
Properties
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Storage instructions
Store at -80°C. Please refer to protocols. -
Components 1 kit ab260606 - Human RIPK2 knockout HeLa cell lysate 1 x 100µg ab255929 - Human wild-type HeLa cell lysate 1 x 100µg -
Research areas
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Cell type
epithelial -
Disease
Adenocarcinoma -
Gender
Female -
STR Analysis
Amelogenin X D5S818: 11, 12 D13S317: 12, 13.3 D7S820: 8, 12 D16S539: 9, 10 vWA: 16, 18 TH01: 7 TPOX: 8, 12 CSF1PO: 9, 10
Images
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Western blot - Human RIPK2 knockout HeLa cell lysate (ab258636)
Lane 1: Wild-type HeLa cell lysate (20 µg)
Lane 2: RIPK2 knockout HeLa cell lysate (20 µg)
Lane 3: Ramos cell lysate (20 µg)
Lane 4: Jurkat cell lysate (20 µg)
Lanes 1-4: Merged signal (red and green). Green - ab75257 observed at 65 kDa. Red - loading control ab181602 observed at 37 kDa.
ab75257 Anti-RIP2 antibody [AF28D3] was shown to specifically react with RIP2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264688 (knockout cell lysate ab258636) was used. Wild-type and RIP2 knockout samples were subjected to SDS-PAGE. ab75257 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 2000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Sanger Sequencing - Human RIPK2 knockout HeLa cell lysate (ab258636)Allele-1: 2 bp deletion in exon 1
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Sanger Sequencing - Human RIPK2 knockout HeLa cell lysate (ab258636)Allele-2: 1 bp insertion in exon 1
