Anti-Hsp60 antibody (ab109660)
Key features and details
- Rabbit polyclonal to Hsp60
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Dog, Human
- Isotype: IgG
Overview
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Product name
Anti-Hsp60 antibody
See all Hsp60 primary antibodies -
Description
Rabbit polyclonal to Hsp60 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Dog, Human -
Immunogen
Recombinant full length Human Hsp60 protein produced in E. coli.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Hsp60 antibody (ab109660)
Immunocytochemistry/Immunofluorescence analysis of Heat Shocked HeLa Cells labeling Hsp60 with ab109660 at 1/100 dilution for 12 hours at 4°C. Cells were fixed in 2% formaldehyde for 20 min at RT. A FITC conjugated Goat Anti-Rabbit secondary antibody (green) was used at 1/200 for 2 hours at RT. DAPI (blue) nuclear counter stain at 1/40000 for 2 hours at RT. Localization in the Mitochondrion matrix observed. (A) DAPI (blue) nuclear stain. (B) Anti-Hsp60 Antibody. (C) Composite.
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Western blot - Anti-Hsp60 antibody (ab109660)All lanes : Anti-Hsp60 antibody (ab109660) at 1 µg/ml
Lane 1 : Recombinant Human Hsp60 at 0.1 µg
Lane 2 : SKBR3 lysate (Human) at 7.5 µg
Lane 3 : MDCK lysate (Dog) at 7.5 µg
Lane 4 : MEF lysate (Mouse) at 7.5 µg
Predicted band size: 61 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
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Immunocytochemistry/ Immunofluorescence - Anti-Hsp60 antibody (ab109660)Immunocytochemistry/Immunofluorescence analysis of Heat Shocked HeLa Cells labeling Hsp60 with ab109660 at 1/100 dilution for 12 hours at 4°C. Cells were fixed in 2% formaldehyde for 20 min at RT. APC Goat Anti-Rabbit (red) secondary antibody was used at 1/200 for 2 hours at RT. DAPI (blue) nuclear counter stain at 1/40000 for 2 hours at RT. Localization in the Mitochondrion matrix observed. (A) DAPI (blue) nuclear stain. (B) Anti-Hsp60 Antibody. (C) Composite.
